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Literature summary for 3.1.3.37 extracted from

  • Feng, L.; Sun, Y.; Deng, H.; Li, D.; Wan, J.; Wang, X.; Wang, W.; Liao, X.; Ren, Y.; Hu, X.
    Structural and biochemical characterization of fructose-1,6/sedoheptulose-1,7-bisphosphatase from the cyanobacterium Synechocystis strain 6803 (2014), FEBS J., 281, 916-926.
    View publication on PubMed
Search for more literature for 3.1.3.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechocystis sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.5, 1.6 M ammonium sulfate, 7-9% (v/v) glycerol Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
D198A inactive Synechocystis sp.
D200A inactive Synechocystis sp.
D33A inactive Synechocystis sp.
D97A inactive Synechocystis sp.
E100A inactive Synechocystis sp.
E225A inactive Synechocystis sp.
E57A inactive Synechocystis sp.
K134A inactive Synechocystis sp.
K29A the mutant shows increased activity compared to the wild type enzyme Synechocystis sp.
R176A inactive Synechocystis sp.
R178G the mutant shows very low activity compared to the wild type enzyme Synechocystis sp.
T102A inactive Synechocystis sp.
Y131A inactive Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 sedoheptulose 1,7-bisphosphate in 50 mM Tris/HCl, pH 8.0, 15 mM MgCl2, 10 mM dithiothreitol, at 289°C Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sedoheptulose 1,7-bisphosphate + H2O Synechocystis sp.
-
 sedoheptulose 7-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. P73922
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA resin column chromatography Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sedoheptulose 1,7-bisphosphate + H2O
-
 Synechocystis sp. sedoheptulose 7-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
FBP/SBPase
-
 Synechocystis sp.
fructose-1,6/sedoheptulose-1,7-bisphosphatase
-
 Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
 sedoheptulose 1,7-bisphosphate in 50 mM Tris/HCl, pH 8.0, 15 mM MgCl2, 10 mM dithiothreitol, at 289°C Synechocystis sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17.5
-
 sedoheptulose 1,7-bisphosphate in 50 mM Tris/HCl, pH 8.0, 15 mM MgCl2, 10 mM dithiothreitol, at 289°C Synechocystis sp.