Application | Comment | Organism |
---|---|---|
molecular biology | catalytically inactive SHIP2 mutant P686A/D690A/R691A reduces preadipocyte proliferation by attenuating PDGFR signaling | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in murine 3T3-L1 preadipocytes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
P686A/D690A/R691A | catalytically inactive dominant-negative mutant inhibits proliferation of preadipocytes more potently than wild-type SHIP2. Phospho-Akt, phospho-ERK1/2, and PDGF receptor (PDGFR) levels are reduced in mutant-expressing preadipocytes. The inhibition of PDGF-activated mitogenic pathways by SHIP2 mutant is consistent with a decrease in PDGFR phosphorylation caused by a drop in receptor levels in SHIP2 mutant-expressing cells. SHIP2 mutant promotes ubiquitination of the PDGFR and its degradation via the lysosomal pathway independently of the association between the E3 ubiquitin ligase c-Cbl and PDGFR | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
SHIP2 | - |
Homo sapiens |