Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hydrogenobacter thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA at 10 mM; not inhibitory: EDTA at 10 mM | Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
L-phosphoserine | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
1.6 | - |
L-phosphoserine | pH 8.0, 70°C | Hydrogenobacter thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent metal ion required | Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 24500, protein PspA, plus 1 * 24000, protein PspB, SDS-PAGE | Hydrogenobacter thermophilus |
24300 | - |
2 * 24300, SDS-PAGE | Hydrogenobacter thermophilus |
24500 | - |
1 * 24500, protein PspA, plus 1 * 24000, protein PspB, SDS-PAGE | Hydrogenobacter thermophilus |
37000 | - |
gel filtration | Hydrogenobacter thermophilus |
46000 | - |
gel filtration | Hydrogenobacter thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | D3DFG8 | - |
- |
Hydrogenobacter thermophilus | D3DFP8 | isoform pspB | - |
Hydrogenobacter thermophilus DSM 6534 | D3DFG8 | - |
- |
Hydrogenobacter thermophilus DSM 6534 | D3DFP8 | isoform pspB | - |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
substrate L-phosphoserine, pH 8.0, 70°C | Hydrogenobacter thermophilus |
58 | - |
substrate L-phosphoserine, pH 8.0, 70°C | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phosphoserine + H2O | - |
Hydrogenobacter thermophilus | L-serine + phosphate | strict substrate specificity for L-phosphoserine | ? | |
L-phosphoserine + H2O | - |
Hydrogenobacter thermophilus DSM 6534 | L-serine + phosphate | strict substrate specificity for L-phosphoserine | ? | |
additional information | poor substrates: D-phosphoserine, DL-phosphothreonine, 3-phosphoglycerate, 4-nitrophenylphosphate, and fructose-6-phosphate. Enzyme does not display phosphoglycerate mutase activity | Hydrogenobacter thermophilus | ? | - |
? | |
additional information | poor substrates: D-phosphoserine, DL-phosphothreonine, 3-phosphoglycerate, 4-nitrophenylphosphate, and fructose-6-phosphate. Enzyme does not display phosphoglycerate mutase activity | Hydrogenobacter thermophilus DSM 6534 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 24500, protein PspA, plus 1 * 24000, protein PspB, SDS-PAGE | Hydrogenobacter thermophilus |
dimer | 2 * 24300, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
pspA | - |
Hydrogenobacter thermophilus |
pspB | - |
Hydrogenobacter thermophilus |