Protein Variants | Comment | Organism |
---|---|---|
Q27A | 1.1fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. The pH-activity profile resembles that of wild-type enzyme with slight increases below pH 4.0 and around pH 6.5 | Aspergillus fumigatus |
Q27G | 2.3fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme.Increase in specific activity is almost constant over entire pH-range, pH 4-8 | Aspergillus fumigatus |
Q27I | 3.1fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Remarkable increase in specific activity around pH 6.5, while having no or only a modest effect in the more acidic pH range. The shape of the pH-profile strongly resembles that of Aspergillus terreus - which also has Leu at postion 27 | Aspergillus fumigatus |
Q27L | 3.4fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Remarkable increase in specific activity around pH 6.5, while having no or only a modest effect in the more acidic pH range. The shape of the pH-profile strongly resembles that of Aspergillus terreus - which also has Leu at postion 27 | Aspergillus fumigatus |
Q27N | 1.7fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Increase in specific activity is almost constant over entire pH-range, pH 4-8 | Aspergillus fumigatus |
Q27P | 1.7fold decrease in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Mutant enzyme has a pronounced tendency to aggregate and precipitate | Aspergillus fumigatus |
Q27S | 1.9fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme | Aspergillus fumigatus |
Q27T | 2.8fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme. Increase in specific activity over the entire pH-range, pH 4-8 | Aspergillus fumigatus |
Q27V | 1.3fold increase in specific activity with phytate at pH 5.0 compared to wild-type enzyme | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphoglycerate + H2O | - |
Aspergillus fumigatus | glycerate + phosphate | - |
? | |
4-nitrophenylphosphate + H2O | - |
Aspergillus fumigatus | 4-nitrophenol + phosphate | - |
? | |
ATP + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
beta-glycerophosphate + H2O | - |
Aspergillus fumigatus | glycerol + phosphate | - |
? | |
fructose 1,6-bisphosphate + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus fumigatus | ? + phosphate | - |
? | |
phenyl phosphate | - |
Aspergillus fumigatus | phenol + phosphate | - |
? | |
phosphoenolpyruvate + H2O | - |
Aspergillus fumigatus | pyruvate + phosphate | - |
? |