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Literature summary for 3.1.3.2 extracted from
- Biochemical characterization of an acid phosphatase from Thermus thermophilus (2010), Biosci. Biotechnol. Biochem., 74, 727-735.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged version expressed in Escherichia coli BL21(DE3) | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | 1 mM: 60% inhibition | Thermus thermophilus |  |
| CuCl2 | - |
Thermus thermophilus | |
| EDTA | - |
Thermus thermophilus | |
| EGTA | - |
Thermus thermophilus | |
| additional information | not inhibited by 10 mM sodium tartrate, 1 mM NaF, 1 mM 2-mercaptoethanol | Thermus thermophilus | |
| ZnCl2 | - |
Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
p-nitrophenyl phosphate | - |
Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Thermus thermophilus | |
| Mg2+ | Ca2+ has a more important role than Mg2+ in enzymatic activities | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28400 | - |
probably tetramer 4 * 28400, SDS-PAGE, calculated | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+), gel filtration | Thermus thermophilus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| zymogram analysis after SDS-PAGE | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 670000 | - |
substrate p-nitrophenyl phosphate | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl phosphate + H2O | substrate preference for p-nitrophenyl phosphate, further substrates: 5-bromo-4-chloro-3-indolyl phosphate, ATP, dATP, dTTP, dGTP, dCTP, less preference for phytate, glycerol-2-phosphate | Thermus thermophilus | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | probably tetramer 4 * 28400, SDS-PAGE, calculated | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid phosphatase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | - |
Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
after 15 min at 70°C: 44% residual activity, after 15 min at 90°C: 22% residual activity | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
acetate buffer | Thermus thermophilus |
| 6.5 | - |
Hepes buffer, pH 6.1-pH 7.2 with Tris buffer | Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | 9.1 | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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