Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.2 extracted from

  • Sharma, S.; Rauk, A.; Juffer, A.H.
    A DFT study on the formation of a phosphohistidine intermediate in prostatic acid phosphatase (2008), J. Am. Chem. Soc., 130, 9708-9716.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Homo sapiens P15309
-
-

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate the dephosphorylation of phosphotyrosine by PAP is a two-step process: the first step involves the transfer of a phosphate group from the substrate to the histidine (His12). A number of quantum models are constructed containing various residues that are thought to play a role in the mechanism. In all these models, the transition state displays an associative character. The transition state is stabilized by three active site arginines (Arg11, Arg15, and Arg79), two of which belong to the conserved motif (RHGXRXP). His12 can act as a nucleophile. The enzyme is further characterized by a His257-Asp258 motif. Asp258 acts as a proton donor which becomes protonated when the substrate enters the binding pocket. The transfer of a proton from Asp258 to the leaving group is possibly mediated by a water molecule in the active site. The importance of His257 in lowering the energy barrier for the nucleophilic attack is shown Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
PAP
-
Homo sapiens
prostate acid phosphatase
-
Homo sapiens