Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15309 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | the dephosphorylation of phosphotyrosine by PAP is a two-step process: the first step involves the transfer of a phosphate group from the substrate to the histidine (His12). A number of quantum models are constructed containing various residues that are thought to play a role in the mechanism. In all these models, the transition state displays an associative character. The transition state is stabilized by three active site arginines (Arg11, Arg15, and Arg79), two of which belong to the conserved motif (RHGXRXP). His12 can act as a nucleophile. The enzyme is further characterized by a His257-Asp258 motif. Asp258 acts as a proton donor which becomes protonated when the substrate enters the binding pocket. The transfer of a proton from Asp258 to the leaving group is possibly mediated by a water molecule in the active site. The importance of His257 in lowering the energy barrier for the nucleophilic attack is shown | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
prostate | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Homo sapiens |
prostate acid phosphatase | - |
Homo sapiens |