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Literature summary for 3.1.3.2 extracted from

  • Kawaguchi, T.; Nakano, T.; Sasagawa, K.; Ohashi, T.; Miura, T.; Komoda, T.
    Tartrate-resistant acid phosphatase 5a and 5b contain distinct sugar moieties (2008), Clin. Biochem., 41, 1245-1249.
    View publication on PubMed
Search for more literature for 3.1.3.2

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
TRAP 5a and 5b are highly purified from human blood and femur extracts, respectively, and after purifying the TRAPs by successive chromatography on a series of lectin affinity columns, the chromatographs obtained are used to estimate the composition of their sugar chain structure. TRAP 5a mainly contains a high-mannose-type sugar chain, while TRAP 5b has multi-antennary complex-type sugar chain. These results indicate that the differences between TRAP 5a and 5b consist not only of a difference in modification by sialic acid but differences in other sugar chain structures Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Homo sapiens
-
femur
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
tartrate-resistant acid phosphatase
-
 Homo sapiens
TRAP 5a
-
 Homo sapiens
TRAP 5b
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Homo sapiens