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Literature summary for 3.1.3.2 extracted from

  • Siddiqua, A.; Rehmat, M.; Saeed, A.; Amin, S.; Naz, R.; Sherazi, M.; Khan, G.M.; Saeed, A.
    Acid phosphatases from the liver of Labeo rohita: purification and characterization (2008), Biol. Pharm. Bull., 31, 802-808.
    View publication on PubMed
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
6-ethylmercaptopurine 1 mM, activity: 281%, isoenzyme 2 Labeo rohita
6-ethylmercaptopurine activity: 108% Labeo rohita
adenine 1 mM, activity: 194%, isoenzyme 2 Labeo rohita
adenosine 1 mM, activity: 134%, isoenzyme 2 Labeo rohita
ADP 1 mM, activity: 118%, isoenzyme 2 Labeo rohita
AMP 1 mM, activity: 104%, isoenzyme 1 Labeo rohita
AMP 1 mM, activity: 162%, isoenzyme 2 Labeo rohita
cAMP 1 mM, activity: 106%, isoenzyme 1 Labeo rohita
cAMP 1 mM, activity: 126%, isoenzyme 2 Labeo rohita
cGMP 1 mM, activity: 106%, isoenzyme 1 Labeo rohita
cGMP 1 mM, activity: 250%, isoenzyme 2 Labeo rohita
GMP 1 mM, activity: 104%, isoenzyme 1 Labeo rohita
GMP 1 mM, activity: 168%, isoenzyme 2 Labeo rohita
guanine 1 mM, activity: 107%, isoenzyme 2 Labeo rohita
guanosine 1 mM, activity: 299%, isoenzyme 2 Labeo rohita

Inhibitors

Inhibitors Comment Organism Structure
inorganic phosphate
-
 Labeo rohita
molybdate
-
 Labeo rohita
pyridoxal 5'-phosphate
-
 Labeo rohita
pyridoxamine 5'-phosphate
-
 Labeo rohita
Tartrate
-
 Labeo rohita
vanadate
-
 Labeo rohita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 4-nitrophenyl phosphate pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0093 Labeo rohita
0.16
-
 Phenyl phosphate pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0076 Labeo rohita
0.18
-
 phosphotyrosine pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0058 Labeo rohita
0.2
-
 flavin mononucleotide pH 5.5, 37°C, isoenzyme 1, Vmax (mmol/min/mg): 0.0082 Labeo rohita
0.23
-
 4-nitrophenyl phosphate pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.038 Labeo rohita
0.31
-
 Phenyl phosphate pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.032 Labeo rohita
0.41
-
 flavin mononucleotide pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.0375 Labeo rohita
1.1
-
 phosphotyrosine pH 5.5, 37°C, isoenzyme 2, Vmax (mmol/min/mg): 0.0154 Labeo rohita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 isoenzyme 1, gel filtration Labeo rohita
18000
-
 isoenzyme 2, gel filtration Labeo rohita
100000
-
 gel filtration Labeo rohita

Organism

Organism UniProt Comment Textmining
Labeo rohita
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using gel filtration and affinity chromatography. Purification factor: 12.5 Labeo rohita
using gel filtration and affinity chromatography. Purification factor: 165 Labeo rohita
using gel filtration and affinity chromatography. Purification factor: 900 Labeo rohita

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Labeo rohita
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
-
 Labeo rohita
6.58
-
 isoenzyme 1 Labeo rohita
35.6
-
 isoenzyme 2 Labeo rohita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O relative hydrolytic rate: 100% Labeo rohita 4-nitrophenol + phosphate
-
 ?
adenosine monophosphate + H2O relative hydrolytic rate: 50% Labeo rohita adenosine + phosphate
-
 ?
adenosine triphosphate + H2O relative hydrolytic rate: 25% Labeo rohita ATP + phosphate
-
 ?
alpha-glycerophosphate + H2O relative hydrolytic rate: 13% Labeo rohita glycerol + phosphate
-
 ?
alpha-glycerophosphate + H2O relative hydrolytic rate: 15% Labeo rohita glycerol + phosphate
-
 ?
alpha-glycerophosphate + H2O relative hydrolytic rate: 19% Labeo rohita glycerol + phosphate
-
 ?
alpha-naphthyl phosphate + H2O relative hydrolytic rate: 3% Labeo rohita 1-naphthol + phosphate
-
 ?
alpha-naphthyl phosphate + H2O relative hydrolytic rate: 8% Labeo rohita 1-naphthol + phosphate
-
 ?
alpha-naphthyl phosphate + H2O relative hydrolytic rate: 88% Labeo rohita 1-naphthol + phosphate
-
 ?
beta-glycerophosphate + H2O relative hydrolytic rate: 2% Labeo rohita glycerol + phosphate
-
 ?
beta-glycerophosphate + H2O relative hydrolytic rate: 70% Labeo rohita glycerol + phosphate
-
 ?
beta-glycerophosphate + H2O relative hydrolytic rate: 8% Labeo rohita glycerol + phosphate
-
 ?
beta-naphthyl phosphate + H2O relative hydrolytic rate: 116% Labeo rohita 2-naphthol + phosphate
-
 ?
beta-naphthyl phosphate + H2O relative hydrolytic rate: 70% Labeo rohita 2-naphthol + phosphate
-
 ?
beta-naphthyl phosphate + H2O relative hydrolytic rate: 81% Labeo rohita 2-naphthol + phosphate
-
 ?
cytidine monophosphate + H2O relative hydrolytic rate: 47% Labeo rohita cytidine + phosphate
-
 ?
D-glucose-1-phosphate + H2O relative hydrolytic rate: 13% Labeo rohita D-glucose + phosphate
-
 ?
D-glucose-6-phosphate + H2O relative hydrolytic rate: 14% Labeo rohita D-glucose + phosphate
-
 ?
flavin mononucleotide + H2O relative hydrolytic rate: 45% Labeo rohita riboflavin + phosphate
-
 ?
flavin mononucleotide + H2O relative hydrolytic rate: 91% Labeo rohita riboflavin + phosphate
-
 ?
flavin mononucleotide + H2O relative hydrolytic rate: 98% Labeo rohita riboflavin + phosphate
-
 ?
guanosine monophosphate + H2O relative hydrolytic rate: 32% Labeo rohita guanosine + phosphate
-
 ?
phenyl phosphate + H2O relative hydrolytic rate: 79% Labeo rohita phenol + phosphate
-
 ?
phenyl phosphate + H2O relative hydrolytic rate: 87% Labeo rohita phenol + phosphate
-
 ?
phenyl phosphate + H2O relative hydrolytic rate: 89% Labeo rohita phenol + phosphate
-
 ?
phosphotyrosine + H2O relative hydrolytic rate: 24% Labeo rohita tyrosine + phosphate
-
 ?
phosphotyrosine + H2O relative hydrolytic rate: 35% Labeo rohita tyrosine + phosphate
-
 ?
phosphotyrosine + H2O relative hydrolytic rate: 73% Labeo rohita tyrosine + phosphate
-
 ?
uridine monophosphate + H2O relative hydrolytic rate: 41% Labeo rohita uridine + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 18000, isoenzyme 1, SDS-PAGE Labeo rohita
monomer 1 * 18000, isoenzyme 2, SDS-PAGE Labeo rohita

Synonyms

Synonyms Comment Organism
acid phosphatase
-
 Labeo rohita
high molecular weight acid phosphatase
-
 Labeo rohita
HM-ACP
-
 Labeo rohita
IF1 isoform 1 Labeo rohita
IF2 isoform 2 Labeo rohita
LM-ACP
-
 Labeo rohita
low molecular weight acid phosphatase
-
 Labeo rohita

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Labeo rohita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Labeo rohita

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0007
-
 vanadate
-
 Labeo rohita
0.0009
-
 molybdate
-
 Labeo rohita
0.013
-
 pyridoxamine 5'-phosphate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
0.017
-
 pyridoxamine 5'-phosphate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
0.02
-
 pyridoxal 5'-phosphate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
0.024
-
 Tartrate
-
 Labeo rohita
0.025
-
 vanadate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
0.08
-
 molybdate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
0.138
-
 vanadate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
0.2
-
 molybdate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
0.208
-
 pyridoxal 5'-phosphate pH 5.5, 37°C, isoenzyme 1 Labeo rohita
1.4
-
 inorganic phosphate pH 5.5, 37°C, isoenzyme 2 Labeo rohita
1.5
-
 inorganic phosphate
-
 Labeo rohita
2.7
-
 inorganic phosphate pH 5.5, 37°C, isoenzyme 1 Labeo rohita