KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.434 | - |
p-nitrophenyl phosphate | estimated Km | Mucor hiemalis | |
1.29 | - |
sodium phytate | estimated Km | Mucor hiemalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
native enzyme, gel filtration | Mucor hiemalis |
45000 | - |
purified enzyme, SDS-PAGE | Mucor hiemalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucor hiemalis | - |
has phytase activity | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Mucor hiemalis |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose CL-6B column chromatography, Sephacryl S-100-HR gel filtration, phenyl agarose column chromatography, and Sephadex G-25 gel filtration | Mucor hiemalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
crude cell extract | Mucor hiemalis |
46.7 | - |
after 14.1fold purification | Mucor hiemalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Mucor hiemalis | 1-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | - |
Mucor hiemalis | 2-naphthol + phosphate | - |
? | |
ADP + H2O | - |
Mucor hiemalis | AMP + phosphate | - |
? | |
AMP + H2O | - |
Mucor hiemalis | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Mucor hiemalis | ADP + phosphate | - |
? | |
calcium phytate + H2O | - |
Mucor hiemalis | ? | - |
? | |
D-glucose 1-phosphate + H2O | - |
Mucor hiemalis | D-glucose + phosphate | - |
? | |
glycerol 2-phosphate + H2O | - |
Mucor hiemalis | glycerol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Mucor hiemalis | p-nitrophenol + phosphate | - |
? | |
phenyl phosphate + H2O | - |
Mucor hiemalis | phenol + phosphate | - |
? | |
sodium phytate + H2O | - |
Mucor hiemalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Mucor hiemalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Mucor hiemalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
retains 64%, 63% and 61% of its original activity after heating for 1, 3 and 5 min, respectively, at 80°C | Mucor hiemalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Mucor hiemalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | retains almost 50% of optimal activity at pH 6.0, drops to 13% residual activity at pH 7.0 | Mucor hiemalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mucor hiemalis | isoelectric focusing | - |
7.8 |