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Literature summary for 3.1.3.2 extracted from
- N-glycosylation sites of plant purple acid phosphatases important for protein expression and secretion in insect cells (2007), Arch. Biochem. Biophys., 461, 247-254.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf9 TriEx insect cells | Phaseolus vulgaris |
| expressed in Sf9 TriEx insect cells | Lupinus luteus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K301N | activity comparable to the wild type enzyme | Phaseolus vulgaris |
| N105S | inactive | Lupinus luteus |
| N105S/N367S | inactive | Lupinus luteus |
| N105S/N367S/N424S | inactive | Lupinus luteus |
| N105S/N424S | inactive | Lupinus luteus |
| N108S | inactive | Phaseolus vulgaris |
| N108S/N170S | inactive | Phaseolus vulgaris |
| N108S/N423S | inactive | Phaseolus vulgaris |
| N108SS/N170S/N423S | inactive | Phaseolus vulgaris |
| N170S | inactive | Phaseolus vulgaris |
| N170S/N423S | inactive | Phaseolus vulgaris |
| N367S | inactive | Lupinus luteus |
| N367S/N424S | inactive | Lupinus luteus |
| N423S | inactive | Phaseolus vulgaris |
| N424S | inactive | Lupinus luteus |
| S110A | reduced activity | Phaseolus vulgaris |
| S110P | reduced activity | Phaseolus vulgaris |
| S110T | activity comparable to the wild type enzyme | Phaseolus vulgaris |
| T107I | reduced activity | Lupinus luteus |
| T107N | reduced activity | Lupinus luteus |
| T107S | activity comparable to the wild type enzyme | Lupinus luteus |
| T172A | inactive | Phaseolus vulgaris |
| T172S | activity comparable to the wild type enzyme | Phaseolus vulgaris |
| T369A | activity comparable to the wild type enzyme | Lupinus luteus |
| T369P | activity comparable to the wild type enzyme | Lupinus luteus |
| T369S | activity comparable to the wild type enzyme | Lupinus luteus |
| T425A | inactive | Phaseolus vulgaris |
| T425P | inactive | Phaseolus vulgaris |
| T425S | activity comparable to the wild type enzyme | Phaseolus vulgaris |
| T426I | reduced activity | Lupinus luteus |
| T426N | reduced activity | Lupinus luteus |
| T426S | activity comparable to the wild type enzyme | Lupinus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus luteus | - |
- |
- |
| Phaseolus vulgaris | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Phaseolus vulgaris |
| glycoprotein | - |
Lupinus luteus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Phaseolus vulgaris | - |
| leaf | - |
Lupinus luteus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | low affinity to ATP | Lupinus luteus | ADP + phosphate | - |
? |  |
| p-nitrophenyl phosphate + H2O | - |
Phaseolus vulgaris | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Lupinus luteus | p-nitrophenol + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | prefers phosphoenolpyruvate as a substrate | Lupinus luteus | pyruvate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAP | - |
Phaseolus vulgaris |
| PAP | - |
Lupinus luteus |
| purple acid phosphatase | - |
Phaseolus vulgaris |
| purple acid phosphatase | - |
Lupinus luteus |
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Release 2023.1 (January 2023)
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