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Literature summary for 3.1.3.2 extracted from
- Purification and characterization of banana fruit acid phosphatase (2001), Planta, 214, 243-249.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | 5 mM, complete inhibition | Musa cavendishii |  |
| Co2+ | 5 mM, 65% inhibition | Musa cavendishii | |
| Cu2+ | 5 mM, 84% inhibition | Musa cavendishii | |
| molybdate | 5 mM, complete inhibition, competitive inhibition | Musa cavendishii | |
| NaF | 5 mM, 60% inhibition, mixed type inhibition | Musa cavendishii | |
| phosphate | 5 mM, 8% inhibition, competitive inhibition | Musa cavendishii | |
| phytate | 5 mM, 31% inhibition, competitive inhibition | Musa cavendishii | |
| vanadate | 5 mM, complete inhibition | Musa cavendishii | |
| Zn2+ | 5 mM, 93% inhibition | Musa cavendishii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.03 | - |
diphosphate | pH 5.8, 25°C | Musa cavendishii | |
| 1.14 | - |
Tetrapolyphosphate | pH 5.8, 25°C | Musa cavendishii | |
| 1.28 | - |
phosphoenolpyruvate | pH 5.8, 25°C | Musa cavendishii | |
| 1.66 | - |
ADP | pH 5.8, 25°C | Musa cavendishii | |
| 1.7 | - |
ATP | pH 5.8, 25°C | Musa cavendishii | |
| 1.73 | - |
UTP | pH 5.8, 25°C | Musa cavendishii | |
| 1.78 | - |
2-naphthyl phosphate | pH 5.8, 25°C | Musa cavendishii | |
| 1.79 | - |
phosphotyrosine | pH 5.8, 25°C | Musa cavendishii | |
| 1.81 | - |
4-nitrophenyl phosphate | pH 5.8, 25°C | Musa cavendishii | |
| 1.98 | - |
CTP | pH 5.8, 25°C | Musa cavendishii | |
| 2.1 | - |
Phenyl phosphate | pH 5.8, 25°C | Musa cavendishii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, activation to 150% of control | Musa cavendishii | |
| Mn2+ | 5 mM, activation to 180% of control | Musa cavendishii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
1 * 40000, SDS-PAGE | Musa cavendishii |
| 41000 | - |
gel filtration | Musa cavendishii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Musa cavendishii | - |
L. cv. Cavendish | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Musa cavendishii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Musa cavendishii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | five days following treatment of unripe green banana fruit with ethylene, extractable APase activity increases 10fold | Musa cavendishii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 745 | - |
- |
Musa cavendishii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-naphthyl phosphate + H2O | - |
Musa cavendishii | 2-naphthol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Musa cavendishii | 4-nitrophenol + phosphate | - |
? | |
| ADP + 2 H2O | - |
Musa cavendishii | adenosine + 2 phosphate | - |
? | |
| ATP + H2O | - |
Musa cavendishii | ? | - |
? | |
| CTP + H2O | - |
Musa cavendishii | ? | - |
? | |
| diphosphate + H2O | - |
Musa cavendishii | 2 phosphate | - |
? | |
| phenyl phosphate + H2O | - |
Musa cavendishii | phenol + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | - |
Musa cavendishii | ? | - |
? | |
| phosphotyrosine + H2O | - |
Musa cavendishii | tyrosine + phosphate | - |
? | |
| tetrapolyphosphate + H2O | - |
Musa cavendishii | ? | - |
? | |
| UTP + H2O | - |
Musa cavendishii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Musa cavendishii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APase | - |
Musa cavendishii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Musa cavendishii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.9 | 6.7 | 50% of maximal activity at pH 4.9 and at pH 6.7 | Musa cavendishii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00008 | - |
molybdate | pH 5.8, 25°C | Musa cavendishii | |
| 0.097 | - |
phosphate | pH 5.8, 25°C | Musa cavendishii | |
| 0.53 | - |
NaF | pH 5.8, 25°C | Musa cavendishii | |
| 0.62 | - |
phytate | pH 5.8, 25°C | Musa cavendishii | |
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