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Literature summary for 3.1.3.2 extracted from

  • Coello, P.
    Purification and characterization of secreted acid phosphatase in phosphorus-deficient Arabidopsis thaliana (2002), Physiol. Plant., 116, 293-298.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
F- 10 mM, 91% inhibition Arabidopsis thaliana
molybdate 10 mM, 98% inhibition Arabidopsis thaliana
additional information not inhibited by tartrate Arabidopsis thaliana
nitrate 10 mM, 9% inhibition Arabidopsis thaliana
phosphate 10 mM, 66% inhibition Arabidopsis thaliana
vanadate 10 mM, 96% inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.124
-
diphosphate pH 4.8, 37°C Arabidopsis thaliana
0.378
-
Polyphosphate pH 4.8, 37°C Arabidopsis thaliana
2.32
-
4-nitrophenyl phosphate pH 4.8, 37°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, stimulation to 142% of control Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana roots respond to the absence of an exogenous phosphate source with an increase in the specific activities of secreted acid phosphatases. The enzyme may have a role in mobilizing organic phosphate in the soil ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Arabidopsis thaliana 4-nitrophenol + phosphate
-
?
ADP + H2O 58% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
?
ATP + H2O 68% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
?
D-fructose 6-phosphate + H2O 17% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana D-fructose + phosphate
-
?
D-glucose 1-phosphate + H2O 8.3% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana D-glucose + phosphate
-
?
diphosphate + H2O 169% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana 2 phosphate
-
?
additional information roots respond to the absence of an exogenous phosphate source with an increase in the specific activities of secreted acid phosphatases. The enzyme may have a role in mobilizing organic phosphate in the soil Arabidopsis thaliana ?
-
?
phosphoenolpyruvate + H2O 83.5% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
?
polyphosphate + H2O 72% of the activity with 4-nitrophenyl phosphate Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
More two proteins of 52000 Da and 63000 Da are detected by SDS-PAGE Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana isoelectric focusing, pH-gradient: pH 3.5-10
-
5.9