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Literature summary for 3.1.3.2 extracted from

  • Ansai, T.; Chen, X.; Barik, S.; Takehara, T.
    Conserved proline residues near the N-terminus are important for enzymatic activity of class A bacterial acid phosphatases (2002), Arch. Biochem. Biophys., 408, 144-146.
    View publication on PubMed
Search for more literature for 3.1.3.2

Protein Variants

Protein Variants Comment Organism
DELTAN47 ratio of turnover number to Km-value is 20.7fold lower than that of wild-type enzyme Prevotella intermedia
DELTAN62 ratio of turnover number to Km-value is more than 5650000fold lower than that of wild-type enzyme Prevotella intermedia
DELTAN88 ratio of turnover number to Km-value is more than 5650000fold lower than that of wild-type enzyme Prevotella intermedia
L51T ratio of turnover number to Km-value is 173.8fold lower than that of wild-type enzyme Prevotella intermedia
P54N ratio of turnover number to Km-value is 1883fold lower than that of wild-type enzyme Prevotella intermedia
P55T ratio of turnover number to Km-value is 753fold lower than that of wild-type enzyme Prevotella intermedia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 4-nitrophenyl phosphate pH 4.9, 37°C, wild-type enzyme Prevotella intermedia
0.75
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme L51T Prevotella intermedia
0.78
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme DELTAN47 Prevotella intermedia
2.32
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme DELTAN88 Prevotella intermedia
3.2
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme P55T Prevotella intermedia
3.46
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme P54N Prevotella intermedia
3.64
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme DELTAN62 Prevotella intermedia

Organism

Organism UniProt Comment Textmining
Prevotella intermedia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Prevotella intermedia 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer wild-type and mutant enzymes Prevotella intermedia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme P54N Prevotella intermedia
0.49
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme L51T and P55T Prevotella intermedia
6.6
-
 4-nitrophenyl phosphate pH 4.9, 37°C, mutant enzyme DELTAN47 Prevotella intermedia
26
-
 4-nitrophenyl phosphate pH 4.9, 37°C, wild-type enzyme Prevotella intermedia