Cloned (Comment) | Organism |
---|---|
expression in High Five insect cells | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | native and recombinant enzyme | Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | native enzyme with an iron-zinc center is not activated upon reduction of the enzyme with ferrous ions and ascorbate, but the activity of recombinant enzyme is increased about 4fold | Phaseolus vulgaris | |
Iron | native enzyme with an iron-zinc center is not activated upon reduction of the enzyme with ferrous ions and ascorbate, but the activity of recombinant enzyme is increased about 4fold | Phaseolus vulgaris | |
Zinc | native enzyme with an iron-zinc center is not activated upon reduction of the enzyme with ferrous ions and ascorbate, but the activity of recombinant enzyme is increased about 4fold | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | O24319 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains five N-linked glycosylation sites at Asn81, Asn109, Asn143, Asn211 and Asn396 | Phaseolus vulgaris |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Phaseolus vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
265.8 | - |
- |
Phaseolus vulgaris |
Synonyms | Comment | Organism |
---|---|---|
kbPAP | - |
Phaseolus vulgaris |
purple acid phosphatase | - |
Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
native and recombinant enzyme | Phaseolus vulgaris |