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Literature summary for 3.1.3.2 extracted from
- A sulfate-dependent acid phosphatase of Thiobacillus thiooxidans. Its partial purification and some properties (1974), J. Biochem., 76, 397-408.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | - |
Acidithiobacillus thiooxidans |  |
| ascorbate | - |
Acidithiobacillus thiooxidans | |
| Cys | - |
Acidithiobacillus thiooxidans | |
| dithiothreitol | - |
Acidithiobacillus thiooxidans | |
| F- | NaF | Acidithiobacillus thiooxidans | |
| molybdate | - |
Acidithiobacillus thiooxidans | |
| NaBH4 | - |
Acidithiobacillus thiooxidans | |
| phosphate | - |
Acidithiobacillus thiooxidans | |
| sodium lauryl sulfate | - |
Acidithiobacillus thiooxidans | |
| Tartrate | 20 mM, 16% inhibition | Acidithiobacillus thiooxidans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
p-nitrophenyl phosphate | - |
Acidithiobacillus thiooxidans | |
| 0.23 | - |
alpha-glycerophosphate | - |
Acidithiobacillus thiooxidans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Acidithiobacillus thiooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus thiooxidans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acidithiobacillus thiooxidans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Acidithiobacillus thiooxidans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 0.25 M ammonium sulfate, stable for more than 1 month | Acidithiobacillus thiooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-glycerophosphate + H2O | - |
Acidithiobacillus thiooxidans | glycerol + phosphate | - |
? | |
| 3'-AMP + H2O | - |
Acidithiobacillus thiooxidans | adenosine + phosphate | - |
? | |
| 5'-AMP + H2O | - |
Acidithiobacillus thiooxidans | adenosine + phosphate | - |
? | |
| ADP + H2O | - |
Acidithiobacillus thiooxidans | AMP + phosphate | - |
? | |
| alpha-glycerophosphate + H2O | - |
Acidithiobacillus thiooxidans | glycerol + phosphate | - |
? | |
| ATP + H2O | - |
Acidithiobacillus thiooxidans | ADP + phosphate | - |
? | |
| D-fructose 1,6-diphosphate + H2O | - |
Acidithiobacillus thiooxidans | ? | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Acidithiobacillus thiooxidans | D-fructose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | - |
Acidithiobacillus thiooxidans | D-ribose + phosphate | - |
? | |
| diphosphate + H2O | - |
Acidithiobacillus thiooxidans | 2 phosphate | - |
? | |
| glucose 1-phosphate + H2O | - |
Acidithiobacillus thiooxidans | glucose + phosphate | - |
? | |
| Glucose 6-phosphate + H2O | - |
Acidithiobacillus thiooxidans | Glucose + phosphate | - |
? | |
| GTP + H2O | - |
Acidithiobacillus thiooxidans | GDP + phosphate | - |
? | |
| O-phosphoethanolamine + H2O | - |
Acidithiobacillus thiooxidans | ethanolamine + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Acidithiobacillus thiooxidans | p-nitrophenol + phosphate | - |
? | |
| phosphocholine + H2O | - |
Acidithiobacillus thiooxidans | choline + phosphate | - |
? | |
| phosphopyruvate + H2O | - |
Acidithiobacillus thiooxidans | pyruvate + phosphate | - |
? | |
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