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Literature summary for 3.1.3.2 extracted from

  • Hara, A.; Sawada, H.; Kato, T.; Nakayama, T.; Yamamoto, H.; Matsumoto, Y.
    Purification and characterization of a purple acid phosphatase from rat spleen (1984), J. Biochem., 95, 67-74.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate maximal activation at 1 mM Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridine
-
Rattus norvegicus
F-
-
Rattus norvegicus
molybdate
-
Rattus norvegicus
o-phenanthroline
-
Rattus norvegicus
phosphate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
ATP
-
Rattus norvegicus
0.87
-
ADP
-
Rattus norvegicus
1.1
-
CTP
-
Rattus norvegicus
1.1
-
UTP
-
Rattus norvegicus
1.2
-
phosphoenolpyruvate
-
Rattus norvegicus
1.2
-
phenolphthalein monophosphate
-
Rattus norvegicus
1.3
-
Phenyl phosphate
-
Rattus norvegicus
1.4
-
1-naphthyl phosphate
-
Rattus norvegicus
1.6
-
Phenolphthalein diphosphate
-
Rattus norvegicus
2
-
p-nitrophenyl phosphate
-
Rattus norvegicus
2
-
alpha-casein
-
Rattus norvegicus
2.2
-
GTP
-
Rattus norvegicus
2.9
-
thiamine diphosphate
-
Rattus norvegicus
3.6
-
4-methylumbelliferyl phosphate
-
Rattus norvegicus
4.2
-
phosvitin
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.1-1.0 mM, activation Rattus norvegicus
Iron contains 2 iron atoms per enzyme molecule Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
gel filtration Rattus norvegicus
33000
-
1 * 33000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
106
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O 37% of the activity with p-nitrophenyl phosphate Rattus norvegicus 1-naphthol + phosphate
-
?
2-glycerophosphate + H2O
-
Rattus norvegicus glycerol + phosphate
-
?
4-methylumbelliferyl phosphate + H2O 82% of the activity with p-nitrophenyl phosphate Rattus norvegicus 4-methylumbelliferone + phosphate
-
?
ADP + H2O 102% of the activity with p-nitrophenyl phosphate Rattus norvegicus AMP + phosphate
-
?
alpha-casein + H2O
-
Rattus norvegicus ?
-
?
ATP + H2O 175% of the activity with p-nitrophenyl phosphate Rattus norvegicus ADP + phosphate
-
?
casein + H2O 9% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
?
CDP + H2O 69% of the activity with p-nitrophenyl phosphate Rattus norvegicus CMP + phosphate
-
?
CTP + H2O 130% of the activity with p-nitrophenyl phosphate Rattus norvegicus CDP + phosphate
-
?
diphosphate + H2O
-
Rattus norvegicus 2 phosphate
-
?
Glucose 6-phosphate + H2O
-
Rattus norvegicus Glucose + phosphate
-
?
GTP + H2O 125% of the activity with p-nitrophenyl phosphate Rattus norvegicus GDP + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Rattus norvegicus p-nitrophenol + phosphate
-
?
phenolphthalein diphosphate + H2O 67% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
?
phenolphthalein monophosphate + H2O 35% of the activity with p-nitrophenyl phosphate Rattus norvegicus phenolphthalein + phosphate
-
?
phenyl phosphate + H2O 52% of the activity with p-nitrophenyl phosphate Rattus norvegicus phenol + phosphate
-
?
phosphoenolpyruvate + H2O
-
Rattus norvegicus pyruvate + phosphate
-
?
phosphopyruvate + H2O 22% of the activity with p-nitrophenyl phosphate Rattus norvegicus pyruvate + phosphate
-
?
phosvitin + H2O 15% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
?
thiamine diphosphate + H2O 76% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
?
UTP + 2 H2O 89% of the activity with p-nitrophenyl phosphate Rattus norvegicus UMP + 2 phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.8
-
Rattus norvegicus