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Literature summary for 3.1.3.2 extracted from

  • Ferens, M.; Morawiecka, B.
    Rye germ acid phosphatase: properties of the enzyme and its activation by lectins (1985), Phytochemistry, 24, 2839-2842.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
concanavalin A activates Secale cereale
lentil lectin activates Secale cereale
soybean agglutinin activates Secale cereale
Wheat germ agglutinin activates Secale cereale

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ uncompetitive Secale cereale
Hg2+ uncompetitive Secale cereale
Zn2+ uncompetitive Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
fructose 1,6-diphosphate
-
Secale cereale
0.84
-
ATP
-
Secale cereale
0.98
-
p-nitrophenyl phosphate
-
Secale cereale
1
-
diphosphate
-
Secale cereale
2.9
-
2-glycerophosphate
-
Secale cereale
5.54
-
phytate
-
Secale cereale

Organism

Organism UniProt Comment Textmining
Secale cereale
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
Secale cereale
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-glycerophosphate + H2O
-
Secale cereale glycerol + phosphate
-
?
ATP + H2O
-
Secale cereale ADP + phosphate
-
?
D-fructose 1,6-diphosphate + H2O
-
Secale cereale ?
-
?
diphosphate + H2O
-
Secale cereale 2 phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Secale cereale p-nitrophenol + phosphate
-
?
phytate + H2O
-
Secale cereale ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
in presence of concanavalin A the enzyme is less sensitive to thermal inactivation Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
hydrolysis of p-nitrophenyl phosphate Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
4.3 7 pH 4.3: about 25% of maximal activity, pH 7.0: about 20% of maximal activity Secale cereale