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Literature summary for 3.1.3.2 extracted from

  • Ching, T.M.; Lin, T.P.; Metzger, R.J.
    Purification and properties of acid phosphatase from plump and shriveled seeds of triticale (1987), Plant Physiol., 84, 789-795.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ascorbate 10 mM, 1.8-2fold activation of ATP hydrolysis Secale cereale x Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
ascorbate 10 mM, hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum
citrate 40 mM, 30% inhibition of ATP hydrolysis of enzyme from plump seed Secale cereale x Triticum aestivum
Cu2+ 1 mM, hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum
EDTA 10 mM Secale cereale x Triticum aestivum
F- 5 mM NaF Secale cereale x Triticum aestivum
FeSO4
-
Secale cereale x Triticum aestivum
Hg2+ 1 mM, inhibition of hydrolysis of p-nitrophenyl phosphate, slight increase of ATP hydrolysis Secale cereale x Triticum aestivum
molybdate
-
Secale cereale x Triticum aestivum
Pb(CH3COO)2 10 mM, strong inhibition of enzyme from plump seed, weak inhibition of enzyme from shriveled seed, hydrolysis of p-nitrophenyl phosphate and ATP Secale cereale x Triticum aestivum
phosphate at 1 mM or above, enzyme from plump seed Secale cereale x Triticum aestivum
Tartrate 10 mM, slight inhibition of hydrolysis of p-nitrophenyl phosphate with enzyme from plump seed, slight activation of ATP hydrolysis, enzyme from plump and shriveled seed Secale cereale x Triticum aestivum
Zn2+ 1 mM, hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
ATP enzyme from plump seed Secale cereale x Triticum aestivum
0.35
-
p-nitrophenyl phosphate enzyme from shriveled seed Secale cereale x Triticum aestivum
0.5
-
ATP enzyme from shriveled seed Secale cereale x Triticum aestivum
0.57
-
p-nitrophenyl phosphate enzyme from plump seed Secale cereale x Triticum aestivum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM CaCl2, slight activation of hydrolysis of ATP, 2.3fold increase of activity with p-nitrophenyl phosphate Secale cereale x Triticum aestivum
Cu2+ 1 mM CuSO4, slight activation of ATP hydrolysis Secale cereale x Triticum aestivum
Mg2+ 10 mM MgCl2, 2-3fold increase of hydrolysis of p-nitrophenyl phosphate, slight increase of hydrolysis of ATP Secale cereale x Triticum aestivum
Zn2+ 1 mM ZnSO4, 1.4-1.5fold activation of ATP hydrolysis Secale cereale x Triticum aestivum

Organism

Organism UniProt Comment Textmining
Secale cereale x Triticum aestivum
-
X Triticosecale Wittmarck
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 12% carbohydrate Secale cereale x Triticum aestivum

Purification (Commentary)

Purification (Comment) Organism
-
Secale cereale x Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
seed shriveled and plump Secale cereale x Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O 19% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 33% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum AMP + phosphate
-
?
ATP + H2O 62% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 64% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum ADP + phosphate
-
?
diphosphate + H2O 86% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 84% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum 2 phosphate
-
?
GTP + H2O 74% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 69% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum GDP + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Secale cereale x Triticum aestivum p-nitrophenol + phosphate
-
?
UTP + 2 H2O 62% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 64% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed Secale cereale x Triticum aestivum UMP + 2 phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
hydrolysis of ATP, enzyme from plump seed Secale cereale x Triticum aestivum
4.8
-
hydrolysis of p-nitrophenyl phosphate Secale cereale x Triticum aestivum
5.5
-
hydrolysis of ATP, enzyme from shriveled seed Secale cereale x Triticum aestivum