Cloned (Comment) | Organism |
---|---|
expression as cellulose-binding module-intein-fusion protein in Escherichia coli strain BL21 Star (DE3) | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 60°C, purified recombinant enzyme | Thermotoga maritima | |
0.12 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 80°C, purified recombinant enzyme | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Thermotoga maritima | - |
D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant cellulose-binding module-intein-fusion enzyme 5.62fold from Escherichia coli strain BL21 Star (DE3) by affinity chromatography on regenerated amorphous cellulose followed by intein self-cleavage | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.13 | - |
pH 7.5, 60°C, purified recombinant enzyme | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Thermotoga maritima | D-fructose 6-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
FBPase | - |
Thermotoga maritima |
fructose-1,6-bisphosphatase | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, half-life is 1330 h | Thermotoga maritima |
80 | - |
purified recombinant enzyme, half-life is 55.6 h | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.57 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 60°C, purified recombinant enzyme | Thermotoga maritima | |
58.7 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 80°C, purified recombinant enzyme | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermotoga maritima |