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Literature summary for 3.1.3.11 extracted from

  • Myung, S.; Wang, Y.; Zhang, Y.
    Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: characterization, metabolite stability, and its implications (2010), Process Biochem., 45, 1882-1887.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression as cellulose-binding module-intein-fusion protein in Escherichia coli strain BL21 Star (DE3) Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
D-fructose 1,6-bisphosphate pH 7.5, 60°C, purified recombinant enzyme Thermotoga maritima
0.12
-
D-fructose 1,6-bisphosphate pH 7.5, 80°C, purified recombinant enzyme Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O Thermotoga maritima
-
D-fructose 6-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant cellulose-binding module-intein-fusion enzyme 5.62fold from Escherichia coli strain BL21 Star (DE3) by affinity chromatography on regenerated amorphous cellulose followed by intein self-cleavage Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.13
-
pH 7.5, 60°C, purified recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Thermotoga maritima D-fructose 6-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
FBPase
-
Thermotoga maritima
fructose-1,6-bisphosphatase
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified recombinant enzyme, half-life is 1330 h Thermotoga maritima
80
-
purified recombinant enzyme, half-life is 55.6 h Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.57
-
D-fructose 1,6-bisphosphate pH 7.5, 60°C, purified recombinant enzyme Thermotoga maritima
58.7
-
D-fructose 1,6-bisphosphate pH 7.5, 80°C, purified recombinant enzyme Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Thermotoga maritima