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Literature summary for 3.1.3.11 extracted from
- glpX gene of Mycobacterium tuberculosis: heterologous expression, purification, and enzymatic characterization of the encoded fructose 1,6-bisphosphatase II (2011), Appl. Biochem. Biotechnol., 164, 1376-1389.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene glpX, expression of functional N-terminally His-tagged enzyme in Escherichia coli strain BL21 (DE3), suncloning in Escherichia coli strain DH5alpha | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Mycobacterium tuberculosis |  |
| Li+ | - |
Mycobacterium tuberculosis | |
| phosphate | - |
Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Mycobacterium tuberculosis | |
| 0.044 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 30°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolutely required | Mycobacterium tuberculosis | |
| Mn2+ | absolutely required | Mycobacterium tuberculosis | |
| additional information | the enzyme activity is dependent on bivalent metal ions, but Co2+, Ni2+, Cu2+, Zn2+, Fe2+ and Ca2+ show no significant activation of FBPase at any specific concentration | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, recombinant His-tagged enzyme, SDS-PAGE, x * 36612.5, recombinant His-tagged enzyme, mass spectrometry | Mycobacterium tuberculosis |
| 127000 | - |
recombinant His-tagged enzyme, gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Mycobacterium tuberculosis | - |
D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
gene glpX or Rv1099c | - |
| Mycobacterium tuberculosis H37Rv | - |
gene glpX or Rv1099c | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 70.7fold from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1300 | - |
purified recombinant His-tagged enzyme, pH 7.7, 30°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Mycobacterium tuberculosis | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 36000, recombinant His-tagged enzyme, SDS-PAGE, x * 36612.5, recombinant His-tagged enzyme, mass spectrometry | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FBPase II | - |
Mycobacterium tuberculosis |
| fructose 1,6-bisphosphatase II | - |
Mycobacterium tuberculosis |
| GlpX | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 30 | purified recombinant His-tagged enzyme, 30 min, completely stable | Mycobacterium tuberculosis |
| 40 | - |
purified recombinant His-tagged enzyme, 30 min, inactivation | Mycobacterium tuberculosis |
| 40 | - |
purified recombinant His-tagged enzyme, 30 min, loss of 65% activity | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
recombinant His-tagged enzyme | Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | activity range, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
pH 7.7, 30°C | Mycobacterium tuberculosis | Li+ | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | fructose 1,6-bisphosphatase II is a key gluconeogenic enzyme | Mycobacterium tuberculosis |
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