Cloned (Comment) | Organism |
---|---|
- |
Neisseria meningitidis |
- |
Lactobacillus delbrueckii subsp. lactis |
Crystallization (Comment) | Organism |
---|---|
T16P mutant, hanging drop vapor diffusion method, using 25% (w/v) PEG, 0.2 M NaCl, and 100 mM bis-Tris (pH 6.0) | Lactobacillus delbrueckii subsp. lactis |
Protein Variants | Comment | Organism |
---|---|---|
D10A | inactive | Neisseria meningitidis |
D10C | inactive | Lactobacillus delbrueckii subsp. lactis |
D10E | inactive | Neisseria meningitidis |
D10N | inactive | Lactobacillus delbrueckii subsp. lactis |
D10S | inactive | Lactobacillus delbrueckii subsp. lactis |
H20A | the mutant shows reduced activity compared to the wild type enzyme | Lactobacillus delbrueckii subsp. lactis |
H20N | the mutant shows reduced activity compared to the wild type enzyme | Lactobacillus delbrueckii subsp. lactis |
H20Q | the mutant shows reduced activity compared to the wild type enzyme | Lactobacillus delbrueckii subsp. lactis |
K76A | the mutant shows reduced activity compared to the wild type enzyme | Lactobacillus delbrueckii subsp. lactis |
T16P | the mutant shows reduced activity compared to the wild type enzyme | Lactobacillus delbrueckii subsp. lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
beta-D-glucose 1,6-bisphosphate | wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C | Neisseria meningitidis | |
0.0048 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme T16P, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
0.031 | - |
beta-D-glucose 1,6-bisphosphate | wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
0.041 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20A, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
0.045 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20Q, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
0.066 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme K76A, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
0.17 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20N, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Neisseria meningitidis | |
Mg2+ | required | Lactobacillus delbrueckii subsp. lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus delbrueckii subsp. lactis | - |
- |
- |
Neisseria meningitidis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neisseria meningitidis |
- |
Lactobacillus delbrueckii subsp. lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose 1,6-bisphosphate + H2O | - |
Neisseria meningitidis | D-glucose 6-phosphate + phosphate | - |
? | |
beta-D-glucose 1,6-bisphosphate + H2O | - |
Lactobacillus delbrueckii subsp. lactis | D-glucose 6-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-PGM | - |
Neisseria meningitidis |
beta-PGM | - |
Lactobacillus delbrueckii subsp. lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20A, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
0.026 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme T16P, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
0.62 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20N, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
1.56 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme K76A, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
20 | - |
beta-D-glucose 1,6-bisphosphate | wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C | Neisseria meningitidis | |
21.9 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20Q, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
175 | - |
beta-D-glucose 1,6-bisphosphate | wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20A, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
4 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20N, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
5 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme T16P, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
20 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme K76A, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
500 | - |
beta-D-glucose 1,6-bisphosphate | mutant enzyme H20Q, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
6000 | - |
beta-D-glucose 1,6-bisphosphate | wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C | Lactobacillus delbrueckii subsp. lactis | |
8000 | - |
beta-D-glucose 1,6-bisphosphate | wild type enzyme, in 50 mM K+HEPES pH 7.0 at 25°C | Neisseria meningitidis |