Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Delta1 | deletion of the first and the first two N-terminal amino acids of FBPase affects neither the kinetic properties of the enzyme nor its association with aldolase | Homo sapiens |
DELTA10 | deletion of the first 10 amino acids: affinity to aldolase is significantly reduced, cooperativitiy of Mg2+ activation is abolished and results in biphasic inhibition of the enzyme by AMP. Truncation lowers affinity of muscle FBPase to aldolase about 14times, making it resemble the liver isozyme, Km (D-fructose 1,6-bisphosphate) similar to wild-type | Homo sapiens |
DELTA2 | deletion of the first and the first two N-terminal amino acids of FBPase affects neither the kinetic properties of the enzyme nor its association with aldolase | Homo sapiens |
DELTA3 | deletion of the first 3 amino acids: kinetic properties similar to wild-type, affinity to aldolase is significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type | Homo sapiens |
DELTA4 | deletion of the first 4 amino acids: kinetic properties similar to wild-type, affinity to aldolase is significantly reduced, mutant shows a lower sensitivity to inhibition by AMP and a little weaker activation by Mg2+, Km (D-fructose 1,6-bisphosphate) similar to wild-type | Homo sapiens |
DELTA5 | deletion of the first 5 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat and activation by Mg2+ are significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type | Homo sapiens |
DELTA6 | deletion of the first 6 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat and activation by Mg2+ are significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type | Homo sapiens |
DELTA7 | deletion of the first 7 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat and activation by Mg2+ are significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type | Homo sapiens |
DELTA8 | deletion of the first 8 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type | Homo sapiens |
additional information | deletion of the first and the first two N-terminal amino acids of FBPase affects neither the kinetic properties of the enzyme not its association with aldolase. The kinetic properties of the mutant with deletion of the first three N-terminal amino acids are entirely the same as these of the wild-type muscle enzyme, its affinity to aldolase is significanltly reduced. The same affinity reduction is observed for the FPase construct lacking the first four N-terminal residues. This protein shows a slight perturbation of its kinetics, a lower sensitivity to inhibition by AMP and a little weaker activation by Mg2+ | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Homo sapiens | |
Ca2+ | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
D-fructose 1,6-bisphosphate | mutant DELTA6 | Homo sapiens | |
0.0014 | - |
D-fructose 1,6-bisphosphate | mutant DELTA8 | Homo sapiens | |
0.0015 | - |
D-fructose 1,6-bisphosphate | mutant DELTA5 | Homo sapiens | |
0.0017 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme | Homo sapiens | |
0.0017 | - |
D-fructose 1,6-bisphosphate | wild-type muscle isoenzyme enzyme | Homo sapiens | |
0.0019 | - |
D-fructose 1,6-bisphosphate | mutant DELTA10 | Homo sapiens | |
0.002 | - |
D-fructose 1,6-bisphosphate | mutant DELTA3 | Homo sapiens | |
0.0021 | - |
D-fructose 1,6-bisphosphate | mutant DELTA4 | Homo sapiens | |
0.0021 | - |
D-fructose 1,6-bisphosphate | mutant DELTA7 | Homo sapiens | |
0.0022 | - |
D-fructose 1,6-bisphosphate | wild-type liver isoenzyme enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | activates | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
muscle | - |
Homo sapiens | - |
skeletal muscle | fibre | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Homo sapiens | D-fructose 6-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FBPase | - |
Homo sapiens |
fructose 1,6-bisphosphatase | liver isoenzyme | Homo sapiens |
fructose 1,6-bisphosphatase | muscle isoenzyme | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
D-fructose 1,6-bisphosphate | mutant DELTA6 | Homo sapiens | |
0.0014 | - |
D-fructose 1,6-bisphosphate | mutant DELTA8 | Homo sapiens | |
0.0015 | - |
D-fructose 1,6-bisphosphate | mutant DELTA5 | Homo sapiens | |
0.0019 | - |
D-fructose 1,6-bisphosphate | mutant DELTA10 | Homo sapiens | |
0.002 | - |
D-fructose 1,6-bisphosphate | mutant DELTA3 | Homo sapiens | |
0.0021 | - |
D-fructose 1,6-bisphosphate | mutant DELTA4 | Homo sapiens | |
0.0021 | - |
D-fructose 1,6-bisphosphate | mutant DELTA7 | Homo sapiens | |
16.7 | - |
D-fructose 1,6-bisphosphate | wild-type liver isoenzyme enzyme | Homo sapiens | |
23.5 | - |
D-fructose 1,6-bisphosphate | wild-type muscle isoenzyme enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00014 | - |
mutant DELTA3 | Homo sapiens | AMP | |
0.00016 | - |
wild-type enzyme | Homo sapiens | AMP | |
0.00016 | - |
wild-type muscle enzyme | Homo sapiens | AMP | |
0.00033 | - |
mutant DELTA4 | Homo sapiens | AMP | |
0.00077 | - |
mutant DELTA4 | Homo sapiens | Ca2+ | |
0.00081 | - |
mutant DELTA5 | Homo sapiens | AMP | |
0.00091 | - |
wild-type muscle enzyme | Homo sapiens | Ca2+ | |
0.00099 | - |
mutant DELTA3 | Homo sapiens | Ca2+ | |
0.00131 | - |
mutant DELTA6 | Homo sapiens | Ca2+ | |
0.00137 | - |
mutant DELTA5 | Homo sapiens | Ca2+ | |
0.00139 | - |
mutant DELTA6 | Homo sapiens | AMP | |
0.00197 | - |
mutant DELTA10 | Homo sapiens | AMP | |
0.00218 | - |
mutant DELTA7 | Homo sapiens | Ca2+ | |
0.00393 | - |
mutant DELTA7 | Homo sapiens | AMP | |
0.00425 | - |
mutant DELTA8 | Homo sapiens | AMP | |
0.00506 | - |
wild-type liver enzyme | Homo sapiens | AMP | |
0.00689 | - |
wild-type liver enzyme, biphasic inhibition by Ca2+ | Homo sapiens | Ca2+ | |
0.00731 | - |
mutant DELTA10 | Homo sapiens | Ca2+ | |
0.934 | - |
wild-type liver enzyme, biphasic inhibition by Ca2+ | Homo sapiens | Ca2+ |