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Literature summary for 3.1.3.11 extracted from

  • Gizak, A.; Maciaszczyk, E.; Dzugaj, A.; Eschrich, K.; Rakus, D.
    Evolutionary conserved N-terminal region of human muscle fructose 1,6-bisphosphatase regulates its activity and the interaction with aldolase (2008), Proteins, 72, 209-216.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
Delta1 deletion of the first and the first two N-terminal amino acids of FBPase affects neither the kinetic properties of the enzyme nor its association with aldolase Homo sapiens
DELTA10 deletion of the first 10 amino acids: affinity to aldolase is significantly reduced, cooperativitiy of Mg2+ activation is abolished and results in biphasic inhibition of the enzyme by AMP. Truncation lowers affinity of muscle FBPase to aldolase about 14times, making it resemble the liver isozyme, Km (D-fructose 1,6-bisphosphate) similar to wild-type Homo sapiens
DELTA2 deletion of the first and the first two N-terminal amino acids of FBPase affects neither the kinetic properties of the enzyme nor its association with aldolase Homo sapiens
DELTA3 deletion of the first 3 amino acids: kinetic properties similar to wild-type, affinity to aldolase is significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type Homo sapiens
DELTA4 deletion of the first 4 amino acids: kinetic properties similar to wild-type, affinity to aldolase is significantly reduced, mutant shows a lower sensitivity to inhibition by AMP and a little weaker activation by Mg2+, Km (D-fructose 1,6-bisphosphate) similar to wild-type Homo sapiens
DELTA5 deletion of the first 5 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat and activation by Mg2+ are significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type Homo sapiens
DELTA6 deletion of the first 6 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat and activation by Mg2+ are significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type Homo sapiens
DELTA7 deletion of the first 7 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat and activation by Mg2+ are significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type Homo sapiens
DELTA8 deletion of the first 8 amino acids: mutant is significantly weaker inhibited by AMP than wild-tpye, affinity to aldolase is significantly reduced, kcat significantly reduced, Km (D-fructose 1,6-bisphosphate) similar to wild-type Homo sapiens
additional information deletion of the first and the first two N-terminal amino acids of FBPase affects neither the kinetic properties of the enzyme not its association with aldolase. The kinetic properties of the mutant with deletion of the first three N-terminal amino acids are entirely the same as these of the wild-type muscle enzyme, its affinity to aldolase is significanltly reduced. The same affinity reduction is observed for the FPase construct lacking the first four N-terminal residues. This protein shows a slight perturbation of its kinetics, a lower sensitivity to inhibition by AMP and a little weaker activation by Mg2+ Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Homo sapiens
Ca2+
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
D-fructose 1,6-bisphosphate mutant DELTA6 Homo sapiens
0.0014
-
D-fructose 1,6-bisphosphate mutant DELTA8 Homo sapiens
0.0015
-
D-fructose 1,6-bisphosphate mutant DELTA5 Homo sapiens
0.0017
-
D-fructose 1,6-bisphosphate wild-type enzyme Homo sapiens
0.0017
-
D-fructose 1,6-bisphosphate wild-type muscle isoenzyme enzyme Homo sapiens
0.0019
-
D-fructose 1,6-bisphosphate mutant DELTA10 Homo sapiens
0.002
-
D-fructose 1,6-bisphosphate mutant DELTA3 Homo sapiens
0.0021
-
D-fructose 1,6-bisphosphate mutant DELTA4 Homo sapiens
0.0021
-
D-fructose 1,6-bisphosphate mutant DELTA7 Homo sapiens
0.0022
-
D-fructose 1,6-bisphosphate wild-type liver isoenzyme enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mg2+ activates Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
muscle
-
Homo sapiens
-
skeletal muscle fibre Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Homo sapiens D-fructose 6-phosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
FBPase
-
Homo sapiens
fructose 1,6-bisphosphatase liver isoenzyme Homo sapiens
fructose 1,6-bisphosphatase muscle isoenzyme Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
D-fructose 1,6-bisphosphate mutant DELTA6 Homo sapiens
0.0014
-
D-fructose 1,6-bisphosphate mutant DELTA8 Homo sapiens
0.0015
-
D-fructose 1,6-bisphosphate mutant DELTA5 Homo sapiens
0.0019
-
D-fructose 1,6-bisphosphate mutant DELTA10 Homo sapiens
0.002
-
D-fructose 1,6-bisphosphate mutant DELTA3 Homo sapiens
0.0021
-
D-fructose 1,6-bisphosphate mutant DELTA4 Homo sapiens
0.0021
-
D-fructose 1,6-bisphosphate mutant DELTA7 Homo sapiens
16.7
-
D-fructose 1,6-bisphosphate wild-type liver isoenzyme enzyme Homo sapiens
23.5
-
D-fructose 1,6-bisphosphate wild-type muscle isoenzyme enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00014
-
mutant DELTA3 Homo sapiens AMP
0.00016
-
wild-type enzyme Homo sapiens AMP
0.00016
-
wild-type muscle enzyme Homo sapiens AMP
0.00033
-
mutant DELTA4 Homo sapiens AMP
0.00077
-
mutant DELTA4 Homo sapiens Ca2+
0.00081
-
mutant DELTA5 Homo sapiens AMP
0.00091
-
wild-type muscle enzyme Homo sapiens Ca2+
0.00099
-
mutant DELTA3 Homo sapiens Ca2+
0.00131
-
mutant DELTA6 Homo sapiens Ca2+
0.00137
-
mutant DELTA5 Homo sapiens Ca2+
0.00139
-
mutant DELTA6 Homo sapiens AMP
0.00197
-
mutant DELTA10 Homo sapiens AMP
0.00218
-
mutant DELTA7 Homo sapiens Ca2+
0.00393
-
mutant DELTA7 Homo sapiens AMP
0.00425
-
mutant DELTA8 Homo sapiens AMP
0.00506
-
wild-type liver enzyme Homo sapiens AMP
0.00689
-
wild-type liver enzyme, biphasic inhibition by Ca2+ Homo sapiens Ca2+
0.00731
-
mutant DELTA10 Homo sapiens Ca2+
0.934
-
wild-type liver enzyme, biphasic inhibition by Ca2+ Homo sapiens Ca2+