Literature summary for 3.1.3.11 extracted from

Ludwig, H.C.; Pardo, F.N.; Asenjo, J.L.; Maureira, M.A.; Yanez, A.J.; Slebe, J.C.
Unraveling multistate unfolding of pig kidney fructose-1,6-bisphosphatase using single tryptophan mutants (2007), FEBS J., 274, 5337-5349.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Sus scrofa

Protein Variants

Protein Variants	Comment	Organism
F16W	mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly, except the loss of AMP cooperativity	Sus scrofa
F219W	mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly	Sus scrofa
F232W	mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly	Sus scrofa
F89W	mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	-	Sus scrofa
D-fructose 2,6-bisphosphate	-	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0043	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F89W	Sus scrofa
0.0046	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F232W	Sus scrofa
0.0048	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, recombinant wild-type enzyme	Sus scrofa
0.005	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F16W	Sus scrofa
0.0059	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, nonrecombinant enzyme	Sus scrofa
0.006	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F219W	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	P00636	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sus scrofa

Renatured (Commentary)

Renatured (Comment)	Organism
the unfolded FBPase mutants in 3.5 M guanidinium chloride are diluted to a concentration of 0.1 M guanidinium chloride the recoveries of enzymatic activity are as follows: 60.9% for F16W FBPase, 57.2% for F89W FBPase, 59.8% for F219W FBPase, and 63.6% for F232W FBPase. These results indicate that the unfolding process is not completely reversible. The reduced reversibility is similar to that observed for nonrecombinant FBPase (65.8%)	Sus scrofa

Renatured (Comment)

Organism

the unfolded FBPase mutants in 3.5 M guanidinium chloride are diluted to a concentration of 0.1 M guanidinium chloride the recoveries of enzymatic activity are as follows: 60.9% for F16W FBPase, 57.2% for F89W FBPase, 59.8% for F219W FBPase, and 63.6% for F232W FBPase. These results indicate that the unfolding process is not completely reversible. The reduced reversibility is similar to that observed for nonrecombinant FBPase (65.8%)

Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	-	Sus scrofa	D-fructose 6-phosphate + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
14.2	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F232W	Sus scrofa
18.1	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F16W	Sus scrofa
18.7	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F219W	Sus scrofa
19.2	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, mutant enzyme F89W	Sus scrofa
19.7	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, recombinant wild-type enzyme	Sus scrofa
20.7	-	D-fructose 1,6-bisphosphate	30°C, pH 7.5, nonrecombinant enzyme	Sus scrofa

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.0007	-	30°C, pH 7.5, mutant enzyme F16W	Sus scrofa	D-fructose 2,6-bisphosphate
0.0007	-	30°C, pH 7.5, mutant enzyme F89W	Sus scrofa	D-fructose 2,6-bisphosphate
0.0009	-	30°C, pH 7.5, nonrecombinant enzyme	Sus scrofa	D-fructose 2,6-bisphosphate
0.001	-	30°C, pH 7.5, recombinant wild-type enzyme	Sus scrofa	D-fructose 2,6-bisphosphate
0.0016	-	30°C, pH 7.5, mutant enzyme F219W	Sus scrofa	D-fructose 2,6-bisphosphate
0.0019	-	30°C, pH 7.5, mutant enzyme F232W	Sus scrofa	D-fructose 2,6-bisphosphate
0.0031	-	30°C, pH 7.5, mutant enzyme F89W	Sus scrofa	AMP
0.0034	-	30°C, pH 7.5, mutant enzyme F219W	Sus scrofa	AMP
0.0046	-	30°C, pH 7.5, mutant enzyme F232W	Sus scrofa	AMP
0.0057	-	30°C, pH 7.5, mutant enzyme F16W	Sus scrofa	AMP
0.0071	-	30°C, pH 7.5, recombinant wild-type enzyme	Sus scrofa	AMP
0.0102	-	30°C, pH 7.5, nonrecombinant enzyme	Sus scrofa	AMP