Crystallization (Comment) | Organism |
---|---|
mutant L54A, in presence of AMP. Growth of crystals in a T-and a R-like conformation | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L54A | enzymic activity similar to wild-type, but much less sensitive to inhibition by AMP. Crystallization data in presence of AMP | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | transformation of enzyme to inactive T-state, inhibition shows quarternary transition and cooperativity | Escherichia coli | |
D-fructose 2,6-bisphosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00094 | - |
D-fructose 1,6-bisphosphate | mutant L54A, 22°C, pH 7.5 | Escherichia coli | |
0.0012 | - |
D-fructose 1,6-bisphosphate | wild-type, 22°C, pH 7.5 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Escherichia coli | D-fructose 6-phosphate + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.3 | - |
D-fructose 1,6-bisphosphate | mutant L54A, 22°C, pH 7.5 | Escherichia coli | |
20 | - |
D-fructose 1,6-bisphosphate | wild-type, 22°C, pH 7.5 | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
activity ratio at pH 7.5 to pH 9.5 is 3.5 for wild-type, 3.9 for mutant L54A | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00023 | - |
D-fructose 2,6-bisphosphate | wild-type, 22°C, pH 7.5 | Escherichia coli | |
0.00038 | - |
D-fructose 2,6-bisphosphate | mutant L54A, 22°C, pH 7.5 | Escherichia coli | |
0.00062 | - |
AMP | wild-type, 22°C, pH 7.5 | Escherichia coli | |
3 | - |
AMP | mutant L54A, 22°C, pH 7.5 | Escherichia coli |