Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.11 extracted from

  • Iancu, C.V.; Mukund, S.; Fromm, H.J.; Honzatko, R.B.
    R-state AMP complex reveals initial steps of the quaternary transition of fructose-1,6-bisphosphatase (2005), J. Biol. Chem., 280, 19737-19745.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant L54A, in presence of AMP. Growth of crystals in a T-and a R-like conformation Escherichia coli

Protein Variants

Protein Variants Comment Organism
L54A enzymic activity similar to wild-type, but much less sensitive to inhibition by AMP. Crystallization data in presence of AMP Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
AMP transformation of enzyme to inactive T-state, inhibition shows quarternary transition and cooperativity Escherichia coli
D-fructose 2,6-bisphosphate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00094
-
D-fructose 1,6-bisphosphate mutant L54A, 22°C, pH 7.5 Escherichia coli
0.0012
-
D-fructose 1,6-bisphosphate wild-type, 22°C, pH 7.5 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Escherichia coli D-fructose 6-phosphate + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.3
-
D-fructose 1,6-bisphosphate mutant L54A, 22°C, pH 7.5 Escherichia coli
20
-
D-fructose 1,6-bisphosphate wild-type, 22°C, pH 7.5 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
activity ratio at pH 7.5 to pH 9.5 is 3.5 for wild-type, 3.9 for mutant L54A Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00023
-
D-fructose 2,6-bisphosphate wild-type, 22°C, pH 7.5 Escherichia coli
0.00038
-
D-fructose 2,6-bisphosphate mutant L54A, 22°C, pH 7.5 Escherichia coli
0.00062
-
AMP wild-type, 22°C, pH 7.5 Escherichia coli
3
-
AMP mutant L54A, 22°C, pH 7.5 Escherichia coli