Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.11 extracted from

  • Rittmann, D.; Schaffer, S.; Wendisch, V.F.; Sahm, H.
    Fructose-1,6-bisphosphatase from Corynebacterium glutamicum: expression and deletion of the fbp gene and biochemical characterization of the enzyme (2003), Arch. Microbiol., 180, 285-292.
    View publication on PubMed
Search for more literature for 3.1.3.11

Cloned(Commentary)

Cloned (Comment) Organism
xpression with a N-terminal His-tag in Escherichia coli Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
AMP-Mn
-
 Corynebacterium glutamicum
D-fructose-1-phosphate
-
 Corynebacterium glutamicum
D-fructose-2,6-bisphosphate
-
 Corynebacterium glutamicum
Li+
-
 Corynebacterium glutamicum
MnAMP
-
 Corynebacterium glutamicum
MnUDP
-
 Corynebacterium glutamicum
phosphate
-
 Corynebacterium glutamicum
phosphoenolpyruvate
-
 Corynebacterium glutamicum
UDP-Mn
-
 Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 D-fructose-1,6-bisphosphate pH 7.7, 30°C Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Corynebacterium glutamicum
Mn2+
-
 Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
 4 * 35500, SDS-PAGE Corynebacterium glutamicum
140000
-
-
 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O Corynebacterium glutamicum enzyme is usually regarded as a regulatory enzyme of gluconeogenesis D-fructose 6-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzyme Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
 Corynebacterium glutamicum D-fructose 6-phosphate + phosphate
-
 ?
D-fructose 1,6-bisphosphate + H2O enzyme is usually regarded as a regulatory enzyme of gluconeogenesis Corynebacterium glutamicum D-fructose 6-phosphate + phosphate
-
 ?
D-glucose 6-phosphate + H2O 23% of the activity found with D-fructose 1,6-bisphosphate Corynebacterium glutamicum D-glucose + phosphate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 35500, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
FBPase II
-
 Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2
-
 D-fructose-1,6-bisphosphate pH 7.7, 30°C Corynebacterium glutamicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.09
-
 AMP-Mn pH 7.7, 30°C Corynebacterium glutamicum
0.14
-
 Li+ pH 7.7, 30°C Corynebacterium glutamicum
0.36
-
 phosphoenolpyruvate pH 7.7, 30°C Corynebacterium glutamicum
1.2
-
 D-fructose-1-phosphate pH 7.7, 30°C Corynebacterium glutamicum
1.6
-
 phosphate pH 7.7, 30°C Corynebacterium glutamicum
3.4
-
 D-fructose-2,6-bisphosphate pH 7.7, 30°C Corynebacterium glutamicum
5
-
 UDP-Mn pH 7.7, 30°C Corynebacterium glutamicum