Protein Variants | Comment | Organism |
---|---|---|
G191A | decreased Km-value for fructose 1,6-diphosphate, decreased inhibition constant for fructose 1,6-diphosphate and decreased Mg2+ affinity compared to the wild type enzyme. The 50% inhibiting concentration of AMP is increased over 2000fold relative to the wild type enzyme, loss of AMP cooperativity, mechanism of AMP inhibition changes from competitive to noncompetitive | Sus scrofa |
I190T | decreased Km-value for fructose 1,6-diphosphate, decreased inhibition constant for fructose 1,6-diphosphate decreased Mg2+ affinity compared to the wild type enzyme. The 50% inhibiting concentration of AMP is increased over 2000fold relative to the wild type enzyme, loss of AMP cooperativity, mechanism of AMP inhibition changes from competitive to noncompetitive | Sus scrofa |
K42E | decreased Km-value for fructose 1,6-diphosphate, decreased inhibition constant for fructose 1,6-diphosphate and decreased Mg2+ affinity compared to the wild type enzyme. The 50% inhibiting concentration of AMP is increased over 2000fold relative to the wild type enzyme, loss of AMP cooperativity | Sus scrofa |
K42T | decreased Km-value for fructose 1,6-diphosphate, decreased inhibition constant for fructose 1,6-diphosphate decreased Mg2+ affinity compared to the wild type enzyme. The 50% inhibiting concentration of AMP is increased over 2000fold relative to the wild type enzyme, loss of AMP cooperativity, mechanism of AMP inhibition changes from competitive to noncompetitive | Sus scrofa |
Q32L | decreased Km-value for fructose 1,6-diphosphate and decreased inhibition constant for fructose 1,6-diphosphate compared to the wild type enzyme. 1.7fold increase in turnover number, 8fold increase in Mg2+ affinity. 8fold increase in 50% inhibiting concentration of AMP | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00084 | - |
fructose 1,6-diphosphate | mutant enzyme G191A | Sus scrofa | |
0.00142 | - |
fructose 1,6-diphosphate | mutant enzyme Q32L | Sus scrofa | |
0.00153 | - |
fructose 1,6-diphosphate | mutant enzyme K42T | Sus scrofa | |
0.00167 | - |
fructose 1,6-diphosphate | mutant enzyme K42E | Sus scrofa | |
0.00181 | - |
fructose 1,6-diphosphate | mutant enzyme I190T | Sus scrofa | |
0.00351 | - |
fructose 1,6-diphosphate | wild type enzyme | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P00636 | - |
- |
Purification (Comment) | Organism |
---|---|
wild type and mutant enzymes | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | - |
Sus scrofa | D-fructose 6-phosphate + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
fructose 1,6-diphosphate | mutant enzyme G191A | Sus scrofa | |
18 | - |
fructose 1,6-diphosphate | wild type enzyme | Sus scrofa | |
18.3 | - |
fructose 1,6-diphosphate | mutant enzyme K42T | Sus scrofa | |
19.3 | - |
fructose 1,6-diphosphate | mutant enzyme K42E | Sus scrofa | |
21.1 | - |
fructose 1,6-diphosphate | mutant enzyme I190T | Sus scrofa | |
32.1 | - |
fructose 1,6-diphosphate | mutant enzyme Q32L | Sus scrofa |