Protein Variants | Comment | Organism |
---|---|---|
D68E | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme | Sus scrofa |
D74A | 50000fold reduced turnover number relative to wild type enzyme | Sus scrofa |
D74E | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme, no AMP cooperativity, kinetic mechanism of AMP inhibition with respect to Mg2+ is changed from competitive to noncompetitive | Sus scrofa |
D74N | 2000fold reduced turnover number relative to wild type enzyme | Sus scrofa |
K71A | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 7.5 for the mutant enzyme | Sus scrofa |
K71M/K72M | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 7.5 for the mutant enzyme, 175fold increased inhibition constant for AMP, 2fold increased affinity for Mg2+ | Sus scrofa |
N64A | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme, no AMP cooperativity, kinetic mechanism of AMP inhibition with respect to Mg2+ is changed from competitive to noncompetitive | Sus scrofa |
N64Q | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00103 | - |
fructose 1,6-diphosphate | mutant enzyme D74E, pH 8.5 | Sus scrofa | |
0.00131 | - |
fructose 1,6-diphosphate | mutant enzyme D68E, pH 7.5 | Sus scrofa | |
0.00142 | - |
fructose 1,6-diphosphate | mutant enzyme N64A, pH 8.5 | Sus scrofa | |
0.0016 | - |
fructose 1,6-diphosphate | mutant enzyme K71M/K72M, pH 7.5 | Sus scrofa | |
0.0017 | - |
fructose 1,6-diphosphate | mutant enzyme N64E, pH 8.5 | Sus scrofa | |
0.0022 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 8.5 | Sus scrofa | |
0.0028 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 7.5 | Sus scrofa | |
0.0028 | - |
fructose 1,6-diphosphate | mutant enzyme K71A, pH 7.5 | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | - |
Sus scrofa | D-fructose 6-phosphate + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
fructose 1,6-diphosphate | mutant enzyme D74A, pH 8.5 | Sus scrofa | |
0.01 | - |
fructose 1,6-diphosphate | mutant enzyme D74N, pH 8.5 | Sus scrofa | |
1.2 | - |
fructose 1,6-diphosphate | mutant enzyme D74E, pH 8.5 | Sus scrofa | |
5.4 | - |
fructose 1,6-diphosphate | mutant enzyme N64E, pH 8.5 | Sus scrofa | |
6.4 | - |
fructose 1,6-diphosphate | mutant enzyme D68E, pH 7.5 | Sus scrofa | |
7.1 | - |
fructose 1,6-diphosphate | mutant enzyme N64A, pH 8.5 | Sus scrofa | |
9.1 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 8.5 | Sus scrofa | |
16 | - |
fructose 1,6-diphosphate | mutant enzyme K71A, pH 7.5 | Sus scrofa | |
21 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 7.5 | Sus scrofa | |
34 | - |
fructose 1,6-diphosphate | mutant enzyme K71M/K72M, pH 7.5 | Sus scrofa |