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Literature summary for 3.1.3.11 extracted from
- Purification of fructose-1,6-diphosphate from Polysphondylium pallidum (1975), Methods Enzymol., 42C, 360-363.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.1-0.2 mM, doubles activity at pH 9-9.5 | Heterostelium pallidum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | no significant inhibition | Heterostelium pallidum | |
| EDTA | 1 mM | Heterostelium pallidum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for divalent metal ion is best satisfied by 1 mM Mg2+ | Heterostelium pallidum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heterostelium pallidum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Heterostelium pallidum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Heterostelium pallidum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several weeks | Heterostelium pallidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Heterostelium pallidum | D-fructose 6-phosphate + phosphate | - |
? | |
| sedoheptulose 1,7-bisphosphate + H2O | - |
Heterostelium pallidum | sedoheptulose 7-phosphate + phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | - |
Heterostelium pallidum |
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