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Literature summary for 3.1.3.11 extracted from
- Fructose-1,6-diphosphate from bovine liver (1975), Methods Enzymol., 42C, 363-368.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-Fluoro-2,4-dinitrobenzene | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus |  |
| 5,5'-dithiobis-(2-nitrobenzoate) | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| nagarse | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| NEM | up to 90% inhibition at pH 6.5-7.0, slight increase of activity at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| p-mercuribenzoate | up to 90% inhibition at pH 6.5-7.0, slight increase of activity at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| papain | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| Pronase | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-Fluoro-2,4-dinitrobenzene | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 5,5'-dithiobis(2-nitrobenzoate) | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| AMP | allosteric | Bos taurus | |
| fructose 1,6-diphosphate | at high concentrations | Bos taurus | |
| Glutaraldehyde | - |
Bos taurus | |
| iodoacetate | - |
Bos taurus | |
| nagarse | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| NEM | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| p-mercuribenzoate | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| papain | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| Pronase | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
fructose 1,6-diphosphate | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | divalent cation required. Mn2+ or Mg2+ | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
equilibrium sedimentation | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | Bos taurus | enzyme in gluconeogenic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.5-8.0, 10 mM Tris, 1.0 mM EDTA, 3.0 mM MgSO4 or 20 mM beta-glycerophosphate, 1.0 mM fructose 1,6-diphosphate, stable for many months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glycerophosphate + H2O | substrate at pH 9.0, no activity at pH 6.5 | Bos taurus | ? | - |
? | |
| D-fructose 1,6-diphosphate + H2O | enzyme in gluconeogenic pathway | Bos taurus | ? | - |
? | |
| D-fructose 1,6-diphosphate + H2O | - |
Bos taurus | D-fructose 6-phosphate + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
fructose 1,6-diphosphate, Mg2+ and Mn2+ protect against thermal inactivation | Bos taurus |
| 50 | - |
15 min, 50% loss of activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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