Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | stimulates | Rhodococcus opacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 0.1 mM, 9% inhibition of isoenzyme A, complete inhibition of isoenzyme B | Rhodococcus opacus | |
ATP | - |
Rhodococcus opacus | |
Mn2+ | inhibition above 0.5 mM | Rhodococcus opacus | |
NAD+ | no inhibition of isoenzyme A, 67% inhibition of isoenzyme B | Rhodococcus opacus | |
NADH | 20% inhibition of isoenzyme A, 83% inhibition of isoenzyme B | Rhodococcus opacus | |
Ribulose 1,5-diphosphate | 1 mM, 48% inhibition of isoenzyme A, 3% inhibition of isoenzyme B | Rhodococcus opacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
sedoheptulose 1,7-diphosphate | isoenzyme A and B | Rhodococcus opacus | |
0.017 | - |
fructose 1,6-diphosphate | isoenzyme A and B | Rhodococcus opacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rhodococcus opacus | |
Mn2+ | can partially replace Mg2+ in activation | Rhodococcus opacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
isoenzyme A, gel filtration | Rhodococcus opacus |
190000 | - |
isoenzyme B, gel filtration | Rhodococcus opacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | Rhodococcus opacus | isoenzyme A is present when the organism is grown under autotrophic conditions but is not synthesized during heterotrophic growth. Isoenzyme B is detected in both autothrophically and heterotrophically grown organisms | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, 2 isoenzymes: A and B | Rhodococcus opacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.75 | - |
isoenzyme B, hydrolysis of sedoheptulose 1,6-diphosphate | Rhodococcus opacus |
3.39 | - |
isoenzyme B, hydrolysis of fructose 1,6-diphosphate | Rhodococcus opacus |
11.82 | - |
isoenzyme A, hydrolysis of fructose 1,6-diphosphate | Rhodococcus opacus |
20.45 | - |
isoenzyme B, hydrolysis of sedoheptulose 1,6-diphosphate | Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | isoenzyme A is present when the organism is grown under autotrophic conditions but is not synthesized during heterotrophic growth. Isoenzyme B is detected in both autothrophically and heterotrophically grown organisms | Rhodococcus opacus | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | - |
Rhodococcus opacus | D-fructose 6-phosphate + phosphate | - |
? | |
sedoheptulose 1,7-bisphosphate + H2O | - |
Rhodococcus opacus | sedoheptulose 7-phosphate + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
isoenzyme A, hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate | Rhodococcus opacus |
8.8 | 9 | isoenzyme B, hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate | Rhodococcus opacus |