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Literature summary for 3.1.3.11 extracted from

  • Springate, C.F.; Stachow, C.S.
    Fructose-bisphosphatase from Rhodopseudomonas palustris (1982), Methods Enzymol., 90, 378-384.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, stimulates Rhodopseudomonas palustris
Cys 1 mM, stimulates Rhodopseudomonas palustris
dithiothreitol 1 mM stimulates Rhodopseudomonas palustris
EDTA
-
Rhodopseudomonas palustris
sulfhydryl reagents 1 mM, stimulate in order of decreasing stimulation activity: glutathione, cysteine, dithiothreitol, 2-mercaptoethanol Rhodopseudomonas palustris

Inhibitors

Inhibitors Comment Organism Structure
AMP no inhibition Rhodopseudomonas palustris
p-hydroxymercuribenzoate strong Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
sedoheptulose 1,7-diphosphate
-
Rhodopseudomonas palustris
0.045
-
fructose 1,6-diphosphate
-
Rhodopseudomonas palustris

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 3.3 mM Rhodopseudomonas palustris
Mg2+ divalent cation required. Mn2+ or Mg2+ Rhodopseudomonas palustris
Mn2+ divalent cation required. Mn2+ or Mg2+ Rhodopseudomonas palustris
Mn2+ Km: 0.078 mM Rhodopseudomonas palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
sucrose density gradient centrifugation Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-diphosphate + H2O Rhodopseudomonas palustris the enzyme may be involved in the regulation of the Calvin-Bassham carbon reduction cycle ?
-
?

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodopseudomonas palustris

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Rhodopseudomonas palustris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhodopseudomonas palustris

Storage Stability

Storage Stability Organism
-20°C, 0.1 mM MnCl2, 1 mM DTT, 0.01 M Tris-HCl buffer, pH 7.4, stable for at least 3 months Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-diphosphate + H2O the enzyme may be involved in the regulation of the Calvin-Bassham carbon reduction cycle Rhodopseudomonas palustris ?
-
?
D-fructose 1,6-diphosphate + H2O
-
Rhodopseudomonas palustris D-fructose 6-phosphate + phosphate
-
?
sedoheptulose 1,7-bisphosphate + H2O at 22.4% of the activity with fructose 1,6-diphosphate Rhodopseudomonas palustris sedoheptulose 7-phosphate + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Rhodopseudomonas palustris

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 pH 7.5: 65% of maximal activity, pH 8.5: optimum Rhodopseudomonas palustris