Literature summary for 3.1.3.10 extracted from

Herter, T.; Berezina, O.V.; Zinin, N.V.; Velikodvorskaya, G.A.; Greiner, R.; Borriss, R.
Glucose-1-phosphatase (AgpE) from Enterobacter cloacae displays enhanced phytase activity (2006), Appl. Microbiol. Biotechnol., 70, 60-64.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Enterobacter cloacae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.31	-	D-glucose 1-phosphate	37°C, pH 5.0	Enterobacter cloacae
0.47	-	D-glucose 6-phosphate	37°C, pH 5.0	Enterobacter cloacae
0.51	-	myo-inositol hexakisphosphate	37°C, pH 5.0	Enterobacter cloacae
0.62	-	D-Fructose 1-phosphate	37°C, pH 5.0	Enterobacter cloacae
0.66	-	D-fructose 6-phosphate	37°C, pH 5.0	Enterobacter cloacae
13	-	p-nitrophenyl phosphate	37°C, pH 5.0	Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	2 * 44000, SDS-PAGE	Enterobacter cloacae
82500	-	gel filtration	Enterobacter cloacae
125000	-	PAGE	Enterobacter cloacae

Organism

Organism	UniProt	Comment	Textmining
Enterobacter cloacae	-	enzyme additionally can act as 3-phytase but is only able to cleave off the third phosphate group of myo-inositol	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
32	-	substrate myo-inositol hexakisphosphate, 37°C, pH 5.0	Enterobacter cloacae
40	-	substrate D-fructose 6-phosphate, 37°C, pH 5.0	Enterobacter cloacae
142	-	substrate D-fructose 1-phosphate, 37°C, pH 5.0	Enterobacter cloacae
146	-	substrate D-glucose 1-phosphate, 37°C, pH 5.0	Enterobacter cloacae
159	-	substrate D-glucose 6-phosphate, 37°C, pH 5.0	Enterobacter cloacae
196	-	substrate D-glucose 1-phosphate, 37°C, pH 5.0	Enterobacter cloacae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1-phosphate + H2O	-	Enterobacter cloacae	D-fructose + phosphate	-	?
D-fructose 6-phosphate + H2O	-	Enterobacter cloacae	D-fructose + phosphate	-	?
D-glucose 1-phosphate + H2O	-	Enterobacter cloacae	D-glucose + phosphate	-	?
D-glucose 6-phosphate + H2O	-	Enterobacter cloacae	D-glucose + phosphate	-	?
myo-inositol hexakisphosphate + H2O	-	Enterobacter cloacae	? + phosphate	enzyme is only able to cleave off the third phosphate group of myo-inositol	?
p-nitrophenyl phosphate + H2O	-	Enterobacter cloacae	p-nitrophenol + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 44000, SDS-PAGE	Enterobacter cloacae

Synonyms

Synonyms	Comment	Organism
AgpE	-	Enterobacter cloacae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
24	-	myo-inositol hexakisphosphate	37°C, pH 5.0	Enterobacter cloacae
30	-	p-nitrophenyl phosphate	37°C, pH 5.0	Enterobacter cloacae
105	-	D-fructose 6-phosphate	37°C, pH 5.0	Enterobacter cloacae
109	-	D-glucose 6-phosphate	37°C, pH 5.0	Enterobacter cloacae
111	-	D-Fructose 1-phosphate	37°C, pH 5.0	Enterobacter cloacae
145	-	D-glucose 1-phosphate	37°C, pH 5.0	Enterobacter cloacae

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Release 2023.1 (January 2023)