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Literature summary for 3.1.3.10 extracted from
- Isolation and properties of glucose-1-phosphatase from mycelia of Pholiota nemako (1998), Biosci. Biotechnol. Biochem., 62, 2251-2253.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)6Mo7O24 | 98% inhibition of 4 -nitrophenyl phosphate hydrolysis by 1.0 mM | Pholiota nameko |  |
| CuSO4 | 55% inhibition of 4-nitrophenyl phosphate hydrolysis by 10 mM | Pholiota nameko | |
| HgCl2 | 99% inhibition of 4-nitrophenyl phosphate hydrolysis by 10 mM | Pholiota nameko | |
| NaF | no inhibition through NaF for the acid phosphatase AP35 | Pholiota nameko | |
| tartaric acid | 36% inhibition of 4-nitrophenyl phosphate hydrolysis by 10 mM | Pholiota nameko | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
4-nitrophenyl phosphate | - |
Pholiota nameko | |
| 4.8 | - |
D-glucose 1-phosphate | - |
Pholiota nameko | |
| 7.9 | - |
D-ribose 5-phosphate | - |
Pholiota nameko | |
| 12.2 | - |
D-Fructose 1-phosphate | - |
Pholiota nameko | |
| 12.3 | - |
phosphoenolpyruvate | - |
Pholiota nameko | |
| 13.4 | - |
Adenosine diphosphate | - |
Pholiota nameko | |
| 14.3 | - |
D-fructose 6-phosphate | - |
Pholiota nameko | |
| 56.7 | - |
Adenosine triphosphate | - |
Pholiota nameko | |
| 85.4 | - |
D-galactose 1-phosphate | - |
Pholiota nameko | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | relative activity in presence of CaCl2 10mM 123% | Pholiota nameko | |
| EDTA | relative activity in presence of EDTA 10mM 104% | Pholiota nameko | |
| NaF | relative activity in presence of NaF 10mM 114% | Pholiota nameko | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Pholiota nameko |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1-phosphate + H2O | Pholiota nameko | - |
D-fructose + phosphate | - |
? | |
| D-fructose 6-phosphate + H2O | Pholiota nameko | - |
D-fructose + phosphate | - |
? | |
| D-galactose 1-phosphate + H2O | Pholiota nameko | - |
D-galactose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | Pholiota nameko | - |
D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | Pholiota nameko | - |
D-glucose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | Pholiota nameko | - |
D-ribose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pholiota nameko | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pholiota nameko |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
with ribose 5-phosphate as substrate, purification procedure described | Pholiota nameko |
| 30 | - |
with nitrophenyl phosphate as substrate, purification procedure described | Pholiota nameko |
| 40 | - |
with phosphoenolpyruvate as substrate | Pholiota nameko |
| 40 | - |
purification procedure described | Pholiota nameko |
| 100 | - |
with D-fructose 6-phosphate as substrate, purification procedure described | Pholiota nameko |
| 130 | - |
purification procedure described | Pholiota nameko |
| 130 | - |
with D-fructose 1-phosphate as substrate | Pholiota nameko |
| 210 | - |
purification procedure described | Pholiota nameko |
| 210 | - |
with adenosine diphosphate as substrate | Pholiota nameko |
| 410 | - |
purification procedure described | Pholiota nameko |
| 410 | - |
with adenosine triphosphate as substrate | Pholiota nameko |
| 2090 | - |
purification procedure described | Pholiota nameko |
| 2090 | - |
with D-galactose 1-phosphate as substrate | Pholiota nameko |
| 2360 | - |
purification procedure described | Pholiota nameko |
| 2360 | - |
with D-glucose 1-phosphate as substrate | Pholiota nameko |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Pholiota nameko | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | - |
Pholiota nameko | AMP + phosphate | - |
? | |
| ATP + H2O | - |
Pholiota nameko | ADP + phosphate | - |
? | |
| D-fructose 1-phosphate + H2O | - |
Pholiota nameko | D-fructose + phosphate | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Pholiota nameko | D-fructose + phosphate | - |
? | |
| D-galactose 1-phosphate + H2O | - |
Pholiota nameko | D-galactose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Pholiota nameko | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Pholiota nameko | D-glucose + phosphate | - |
? | |
| D-ribose 5-phosphate + H2O | - |
Pholiota nameko | D-ribose + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | - |
Pholiota nameko | pyruvate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid phosphatase | - |
Pholiota nameko |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
with 4-nitrophenyl phosphate as substrate | Pholiota nameko |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
ribose 5-phosphate | - |
Pholiota nameko | |
| 0.000667 | - |
phosphoenolpyruvate | - |
Pholiota nameko | |
| 0.00167 | - |
fructose 6-phosphate | - |
Pholiota nameko | |
| 0.00217 | - |
fructose 1-phosphate | - |
Pholiota nameko | |
| 0.0035 | - |
ADP | - |
Pholiota nameko | |
| 0.0348 | - |
Galactose 1-phosphate | - |
Pholiota nameko | |
| 0.0393 | - |
glucose 1-phosphate | - |
Pholiota nameko | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
purified enzyme | Pholiota nameko |
| 5.9 | - |
with 4-nitrophenyl phosphate 15 mM, as substrate | Pholiota nameko |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | purified enzyme, with 4-nitrophenyl phosphate 15 mM as substrate, activity rapidly lost above pH8 | Pholiota nameko |
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