Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenate | 1 mM Na2HAsO4, 80% inhibition of isoenzyme I, 22% inhibition of isoenzyme II, 40% inhibition of isoenzyme III | Aspergillus oryzae | |
Ba2+ | 1 mM, 95% inhibition of isoenzyme I | Aspergillus oryzae | |
Borate | 1 mM H3BO4, 16% inhibition of isoenzyme I, 13% inhibition of isoenzyme II, 8% inhibition of isoenzyme III | Aspergillus oryzae | |
Ca2+ | weak inhibition of isoenzyme II | Aspergillus oryzae | |
Cu2+ | 1 mM, 87% inhibition of isoenzyme I, 10% inhibition of isoenzyme III | Aspergillus oryzae | |
EDTA | inhibition of isoenzyme I and II | Aspergillus oryzae | |
F- | 1 mM NaF, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II | Aspergillus oryzae | |
Hg2+ | 1 mM nearly complete inhibition of isoenzyme I, II and III | Aspergillus oryzae | |
Mg2+ | weak inhibition of isoenzyme I | Aspergillus oryzae | |
Mn2+ | 1 mM 90% inhibition of isoenzyme I, 64% inhibition of isoenzyme II, 39% inhibition of isoenzyme III | Aspergillus oryzae | |
NaWO4 | 1 mM NaWO4, 35% inhibition of isoenzyme I, 80% inhibition of isoenzyme II, 14% inhibition of isoenzyme III | Aspergillus oryzae | |
PCMB | 1 mM, 54% inhibition of isoenzyme I, 33% inhibition of isoenzyme II, 9% inhibition of isoenzyme III | Aspergillus oryzae | |
phosphate | 1 mM Na2HPO4, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II, 2% inhibition of isoenzyme III; competitive | Aspergillus oryzae | |
Sr2+ | 1 mM, 88% inhibition of isoenzyme I | Aspergillus oryzae | |
Zn2+ | 1 mM, 98% inhibition of isoenzyme I | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
p-nitrophenyl phosphate | isoenzyme III | Aspergillus oryzae | |
0.22 | - |
p-nitrophenyl phosphate | isoenzyme II | Aspergillus oryzae | |
2 | - |
p-nitrophenyl phosphate | isoenzyme I | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates isoenzyme I, weak activation of isoenzyme III | Aspergillus oryzae | |
Mg2+ | activation of isoenzyme II and III | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
three isoenzymes: I, II, III | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-CMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
2',3'-GMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
2',3-AMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
2-phosphoglycerate + H2O | hydrolyzed by isoenzyme III, weak activity with isoenzyme I and II | Aspergillus oryzae | glycerol + phosphate | - |
? | |
3-phosphoglycerate + H2O | hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I | Aspergillus oryzae | glycerol + phosphate | - |
? | |
5'-AMP + H2O | - |
Aspergillus oryzae | adenosine + phosphate | - |
? | |
5'-dCMP + H2O | - |
Aspergillus oryzae | ? | - |
? | |
5'-GMP + H2O | - |
Aspergillus oryzae | guanosine + phosphate | - |
? | |
alpha-naphthyl phosphate + H2O | hydrolyzed by isoenzyme II and III, no activity with isoenzyme I | Aspergillus oryzae | 1-naphthol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | hydrolyzed by isoenzyme I, II or III | Aspergillus oryzae | p-nitrophenol + phosphate | - |
? | |
phosphoethanolamine + H2O | hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I | Aspergillus oryzae | phosphate + ethanolamine | - |
? | |
phosphorylcholine + H2O | hydrolyzed by isoenzyme III, no activity with isoenzyme I and II | Aspergillus oryzae | phosphate + choline | - |
? | |
phosphoserine + H2O | hydrolyzed by isoenzyme II, weak activity with isoenzyme I and II | Aspergillus oryzae | phosphate + Ser | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 8, 120 min, stable | Aspergillus oryzae |
35 | - |
pH 10, isoenzyme III, 120 min, stable | Aspergillus oryzae |
40 | - |
pH 9-9.5, 120 min, stable | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
isoenzyme I | Aspergillus oryzae |
9 | 9.5 | isoenzyme II | Aspergillus oryzae |
10 | - |
isoenzyme III | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 30°C, stable | Aspergillus oryzae |