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Literature summary for 3.1.3.1 extracted from

  • Sakurai, Y.; Toda, K.; Shiota, H.
    Multiple forms and some properties of alkaline phosphatase produced by Aspergillus oryzae on solid medium (1981), Agric. Biol. Chem., 45, 1959-1967.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
arsenate 1 mM Na2HAsO4, 80% inhibition of isoenzyme I, 22% inhibition of isoenzyme II, 40% inhibition of isoenzyme III Aspergillus oryzae
Ba2+ 1 mM, 95% inhibition of isoenzyme I Aspergillus oryzae
Borate 1 mM H3BO4, 16% inhibition of isoenzyme I, 13% inhibition of isoenzyme II, 8% inhibition of isoenzyme III Aspergillus oryzae
Ca2+ weak inhibition of isoenzyme II Aspergillus oryzae
Cu2+ 1 mM, 87% inhibition of isoenzyme I, 10% inhibition of isoenzyme III Aspergillus oryzae
EDTA inhibition of isoenzyme I and II Aspergillus oryzae
F- 1 mM NaF, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II Aspergillus oryzae
Hg2+ 1 mM nearly complete inhibition of isoenzyme I, II and III Aspergillus oryzae
Mg2+ weak inhibition of isoenzyme I Aspergillus oryzae
Mn2+ 1 mM 90% inhibition of isoenzyme I, 64% inhibition of isoenzyme II, 39% inhibition of isoenzyme III Aspergillus oryzae
NaWO4 1 mM NaWO4, 35% inhibition of isoenzyme I, 80% inhibition of isoenzyme II, 14% inhibition of isoenzyme III Aspergillus oryzae
PCMB 1 mM, 54% inhibition of isoenzyme I, 33% inhibition of isoenzyme II, 9% inhibition of isoenzyme III Aspergillus oryzae
phosphate 1 mM Na2HPO4, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II, 2% inhibition of isoenzyme III; competitive Aspergillus oryzae
Sr2+ 1 mM, 88% inhibition of isoenzyme I Aspergillus oryzae
Zn2+ 1 mM, 98% inhibition of isoenzyme I Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
p-nitrophenyl phosphate isoenzyme III Aspergillus oryzae
0.22
-
p-nitrophenyl phosphate isoenzyme II Aspergillus oryzae
2
-
p-nitrophenyl phosphate isoenzyme I Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates isoenzyme I, weak activation of isoenzyme III Aspergillus oryzae
Mg2+ activation of isoenzyme II and III Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
three isoenzymes: I, II, III
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-CMP + H2O
-
Aspergillus oryzae ?
-
?
2',3'-GMP + H2O
-
Aspergillus oryzae ?
-
?
2',3-AMP + H2O
-
Aspergillus oryzae ?
-
?
2-phosphoglycerate + H2O hydrolyzed by isoenzyme III, weak activity with isoenzyme I and II Aspergillus oryzae glycerol + phosphate
-
?
3-phosphoglycerate + H2O hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I Aspergillus oryzae glycerol + phosphate
-
?
5'-AMP + H2O
-
Aspergillus oryzae adenosine + phosphate
-
?
5'-dCMP + H2O
-
Aspergillus oryzae ?
-
?
5'-GMP + H2O
-
Aspergillus oryzae guanosine + phosphate
-
?
alpha-naphthyl phosphate + H2O hydrolyzed by isoenzyme II and III, no activity with isoenzyme I Aspergillus oryzae 1-naphthol + phosphate
-
?
p-nitrophenyl phosphate + H2O hydrolyzed by isoenzyme I, II or III Aspergillus oryzae p-nitrophenol + phosphate
-
?
phosphoethanolamine + H2O hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I Aspergillus oryzae phosphate + ethanolamine
-
?
phosphorylcholine + H2O hydrolyzed by isoenzyme III, no activity with isoenzyme I and II Aspergillus oryzae phosphate + choline
-
?
phosphoserine + H2O hydrolyzed by isoenzyme II, weak activity with isoenzyme I and II Aspergillus oryzae phosphate + Ser
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 8, 120 min, stable Aspergillus oryzae
35
-
pH 10, isoenzyme III, 120 min, stable Aspergillus oryzae
40
-
pH 9-9.5, 120 min, stable Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
isoenzyme I Aspergillus oryzae
9 9.5 isoenzyme II Aspergillus oryzae
10
-
isoenzyme III Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 30°C, stable Aspergillus oryzae