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Literature summary for 3.1.3.1 extracted from
- Heat-labile alkaline phosphatase from antarctic bacteria: rapid 5' end-labeling of nucleic acids (1984), Proc. Natl. Acad. Sci. USA, 81, 6691-6695.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | antarctic bacterium |
| 68000 | - |
1 * 68000, SDS-PAGE after treatment with 2-mercaptoethanol | antarctic bacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| antarctic bacterium | - |
- |
- |
| antarctic bacterium HK47 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
antarctic bacterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90.3 | - |
- |
antarctic bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
antarctic bacterium | ? | - |
? |  |
| ADP + H2O | - |
antarctic bacterium HK47 | ? | - |
? | |
| AMP + H2O | - |
antarctic bacterium | adenosine + phosphate | - |
? | |
| AMP + H2O | - |
antarctic bacterium HK47 | adenosine + phosphate | - |
? | |
| GTP + H2O | - |
antarctic bacterium | ? | - |
? | |
| GTP + H2O | - |
antarctic bacterium HK47 | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
antarctic bacterium | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
antarctic bacterium HK47 | p-nitrophenol + phosphate | - |
? | |
| UTP + H2O | - |
antarctic bacterium | ? | - |
? | |
| UTP + H2O | - |
antarctic bacterium HK47 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 68000, SDS-PAGE after treatment with 2-mercaptoethanol | antarctic bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
antarctic bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | 10°C: about 30% of maximal activity, 40°C: about 10% of maximal activity | antarctic bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | - |
10 min, little loss of activity | antarctic bacterium |
| 15 | - |
10 min, 40% loss of activity | antarctic bacterium |
| 40 | - |
half-life: 2 min | antarctic bacterium |
| 55 | - |
10 min, complete and irreversible inactivation | antarctic bacterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
0°C, rapid and irreversible loss of activity at or below | antarctic bacterium |
| 7 | 9.5 | 0°C, stable | antarctic bacterium |
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Release 2023.1 (January 2023)
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