Application | Comment | Organism |
---|---|---|
synthesis | overexpression of enzyme in Escherichia coli. After 1 day of growth, when the Escherichia coli culture is near the stationary phase, high amounts of enzyme are produced but the alkaline phosphatase activity in the cell-free extract is near the background level. Further incubation of bacterial culture leads to a tremendous increase in alkaline phosphatase activity which is maximal at the 3rd day of incubation and was 48100 times higher than at the 1st day of growth. Typically, 90-140 mg of active protein is produced in 1 l of culture | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required. In the absence of Co2+ in the lysis buffer, activity of extracts is only 10% of the normal value | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P19406 | - |
- |
Bacillus subtilis 168 | P19406 | - |
- |
Purification (Comment) | Organism |
---|---|
buffers need to be supplemented with 0.1 mM CoCl2. If Co2+ is omitted in the lysis buffer, activity of the cell-free extracts is only about 10% of the normal value. Addition of the absent metal to these extracts restores 100% activity, after overnight incubation at 4°C | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
515 | - |
pH 11, 25°C, presence of 0.8-2 M NaCl | Bacillus subtilis |
Storage Stability | Organism |
---|---|
-80°C, stable for at least one month | Bacillus subtilis |
4°C, cell-free extract, stable for two weeks | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Bacillus subtilis | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Bacillus subtilis 168 | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase 4 | - |
Bacillus subtilis |
alkaline phosphatase IV | - |
Bacillus subtilis |
PHOA | - |
Bacillus subtilis |