Literature summary for 3.1.3.1 extracted from

Koksharov, M.; Lv, C.; Zhai, X.; Ugarova, N.; Huang, E.
Bacillus subtilis alkaline phosphatase IV acquires activity only late at the stationary phase when produced in Escherichia coli. Overexpression and characterization of the recombinant enzyme (2013), Protein Expr. Purif., 90, 186-194.

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Application

Application	Comment	Organism
synthesis	overexpression of enzyme in Escherichia coli. After 1 day of growth, when the Escherichia coli culture is near the stationary phase, high amounts of enzyme are produced but the alkaline phosphatase activity in the cell-free extract is near the background level. Further incubation of bacterial culture leads to a tremendous increase in alkaline phosphatase activity which is maximal at the 3rd day of incubation and was 48100 times higher than at the 1st day of growth. Typically, 90-140 mg of active protein is produced in 1 l of culture	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Bacillus subtilis	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	required. In the absence of Co2+ in the lysis buffer, activity of extracts is only 10% of the normal value	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P19406	-	-
Bacillus subtilis 168	P19406	-	-

Purification (Commentary)

Purification (Comment)	Organism
buffers need to be supplemented with 0.1 mM CoCl2. If Co2+ is omitted in the lysis buffer, activity of the cell-free extracts is only about 10% of the normal value. Addition of the absent metal to these extracts restores 100% activity, after overnight incubation at 4°C	Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
515	-	pH 11, 25°C, presence of 0.8-2 M NaCl	Bacillus subtilis

Storage Stability

Storage Stability	Organism
-80°C, stable for at least one month	Bacillus subtilis
4°C, cell-free extract, stable for two weeks	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Bacillus subtilis	4-nitrophenol + phosphate	-	?
4-nitrophenyl phosphate + H2O	-	Bacillus subtilis 168	4-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
alkaline phosphatase 4	-	Bacillus subtilis
alkaline phosphatase IV	-	Bacillus subtilis
PHOA	-	Bacillus subtilis

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Release 2023.1 (January 2023)