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Literature summary for 3.1.3.1 extracted from

  • Wende, A.; Johansson, P.; Vollrath, R.; Dyall-Smith, M.; Oesterhelt, D.; Grininger, M.
    Structural and biochemical characterization of a halophilic archaeal alkaline phosphatase (2010), J. Mol. Biol., 400, 52-62.
    View publication on PubMed
Search for more literature for 3.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
Tris dephosphorylation of substrate is doubled when the Tris concentration in the reaction buffer is increased from 20 mM to 500 mM Halobacterium salinarum

Crystallization (Commentary)

Crystallization (Comment) Organism
diffracting crystals are grown using the hanging-drop vapor diffusion method. Crystallographic data are collected to a resolution of 1.7 A Halobacterium salinarum

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
 Halobacterium salinarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 4-nitrophenyl phosphate pH 10.5, 20°C, 4 M NaCl Halobacterium salinarum

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl assays at 4 M NaCl Halobacterium salinarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45300
-
 predicted from cDNA Halobacterium salinarum
50000
-
 mass spectrometric analysis of SDS-PAGE-separated protein Halobacterium salinarum
110000
-
 mass spectrometric analysis of SDS-PAGE-separated protein Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum B0R9W3
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HiLoad 16/60 Superdex 200 prep-grade column Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Halobacterium salinarum 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer enzyme forms dimers composed of dominant beta-sheet structures sandwiched by alpha-helices. Active sites are well-accessible Halobacterium salinarum

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Halobacterium salinarum
HSAP
-
 Halobacterium salinarum
OE5192R
-
 Halobacterium salinarum
OE5192R gene coding for HSAP Halobacterium salinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 determination of kinetic constants at Halobacterium salinarum
37
-
 all other experiments at Halobacterium salinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Halobacterium salinarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 stability impaired at pH 10.5. Highest stability at pH 7.5 Halobacterium salinarum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.56
-
 phosphate pH 10.5, 20°C, 4 M NaCl Halobacterium salinarum

General Information

General Information Comment Organism
malfunction deletion of OE5192R produces a mutant strain with virtually no phosphatase activity Halobacterium salinarum