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Literature summary for 3.1.3.1 extracted from

  • Yang, Z.; Liu, X.; Chen, C.; Halling, P.
    Hofmeister effects on activity and stability of alkaline phosphatase (2010), Biochim. Biophys. Acta, 1804, 821-828.
    View publication on PubMed
Search for more literature for 3.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
KCl reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
KNO3 reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
KPF6 reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
Na2SO4 reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
NaBF4 reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
NaCl reduces enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
NaNO3 reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
Sodium acetate reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus
Sodium citrate reduced enzymatic activity and significantly decreased Vmax/Km ratio Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
 4-nitrophenyl phosphate pH 10, 37°C, Vmax: 0.02 mM/min Bos taurus
1.37
-
 4-nitrophenyl phosphate pH not specified, 37°C, Vmax: 0.011 mM/min, in the presence of 0.8 M NaN3 Bos taurus
1.91
-
 4-nitrophenyl phosphate pH 9.5, 37°C, Vmax: 0.029 mM/min, in the presence of 0.8 M KNO3 Bos taurus
2.52
-
 4-nitrophenyl phosphate pH 9.5, 37°C, Vmax: 0.023 mM/min, in the presence of 0.8 M KCl Bos taurus
2.54
-
 4-nitrophenyl phosphate pH 10, 37°C, Vmax: 0.018 mM/min, in the presence of 0.8 M NaAc Bos taurus
2.73
-
 4-nitrophenyl phosphate pH 9.25, 37°C, Vmax: 0.02 mM/min, in the presence of 0.8 M KPF6 Bos taurus
3.43
-
 4-nitrophenyl phosphate pH 9.25, 37°C, Vmax: 0.021 mM/min, in the presence of 0.8 M NaNO3 Bos taurus
4.4
-
 4-nitrophenyl phosphate pH 9, 37°C, Vmax: 0.016 mM/min, in the presence of 0.8 M NaBF4 Bos taurus
4.42
-
 4-nitrophenyl phosphate pH 9.5, 37°C, Vmax: 0.028 mM/min, in the presence of 0.8 M NaCl Bos taurus
6.87
-
 4-nitrophenyl phosphate pH 9, 37°C, Vmax: 0.022 mM/min, in the presence of 0.8 M Na2SO4 Bos taurus
7.32
-
 4-nitrophenyl phosphate pH not specified, 37°C, Vmax: 0.019 mM/min, in the presence of 0.8 M Na citrate Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
sulfate the rate of irreversible thermal inactivation is strongly reduced by addition of kosmotropic anions like sulfate (half-life increases from 8 to 580 min at 60 °C) Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Bos taurus 4-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the half-lives for the enzyme incubated in saltfree buffer at 50°C, 60°C, and 70°C is determined to be 5.3 h, 7.9 min, and 1.7 min. All the salts tested show the ability to stabilize the enzyme when incubated at 70°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
 assay at Bos taurus