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Literature summary for 3.1.3.1 extracted from

  • Sakar, A.; Hess, D.; Mondal, H.; Banerjee, S.; Sharma, H.; Das, S.
    Homodimeric alkaline phosphatase located at Helicoverpa armigera midgut, a putative receptor of Cry1Ac contains -GalNAc in terminal glycan structure as interactive epitope (2009), J. Proteome Res., 8, 1838-1848.
    View publication on PubMed
Search for more literature for 3.1.3.1

Protein Variants

Protein Variants Comment Organism
additional information HaALP is identified in the target insect gut brush border membrane vesicles as a receptor of the Bacillus thuringiensis coded Cry1Ac toxin. Lectin-ligand immunoblot assay detects the presence of alpha-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction Helicoverpa armigera

Inhibitors

Inhibitors Comment Organism Structure
Cry1Ac toxin environment friendly insecticidal crystal proteins encoded by the spore-forming bacteria Bacillus thuringiensis. Competitive inhibitor; environment friendly insecticidal crystal proteins encoded by the spore-forming bacteria Bacillus thuringiensis. Competitive inhibitor Helicoverpa armigera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.58
-
 4-nitrophenyl phosphate
-
 Helicoverpa armigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 2 * 68000, SDS-PAGE Helicoverpa armigera
138000
-
 gel filtration Helicoverpa armigera

Organism

Organism UniProt Comment Textmining
Helicoverpa armigera B4Z1D6
-
-
Helicoverpa armigera B4Z1D7
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration chromatography using a pre-equilibrated column containing Sephadex G-150 Helicoverpa armigera

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Helicoverpa armigera 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 68000, SDS-PAGE Helicoverpa armigera

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Helicoverpa armigera
HaALP
-
 Helicoverpa armigera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Helicoverpa armigera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.14
-
-
 Helicoverpa armigera
10.3
-
 assay at Helicoverpa armigera

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000316
-
 Cry1Ac toxin competitive inhibition Helicoverpa armigera