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Literature summary for 3.1.3.1 extracted from

  • Cho-Ngwa, F.; Mbua, E.N.; Nchamukong, K.G.; Titanji, V.P.
    Detection, purification and characterisation of a secretory alkaline phosphatase from Onchocerca species (2007), Mol. Biochem. Parasitol., 156, 136-143.
    View publication on PubMed

General Stability

General Stability Organism
requires Mg2+ and dithiothreitol for stability throughout its purification Onchocerca ochengi
sodium dodecyl sulphate at 2% (w/v) stabilises the enzyme during freezing Onchocerca ochengi

Inhibitors

Inhibitors Comment Organism Structure
HPO42- competitive Onchocerca ochengi
L-Phe mixed-type inhibition Onchocerca ochengi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
4-nitrophenyl phosphate 37°C, pH 9.8 Onchocerca ochengi

Organism

Organism UniProt Comment Textmining
Onchocerca ochengi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Onchocerca ochengi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.86
-
-
Onchocerca ochengi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Onchocerca ochengi 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
dimer the 90000 Da monomeric species dimerises to about 180000 Da upon purification Onchocerca ochengi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.2
-
-
Onchocerca ochengi

pH Range

pH Minimum pH Maximum Comment Organism
9 11 pH 9.0: about 35% of maximal activity, pH 11.0: about 35% of maximal activity Onchocerca ochengi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.18
-
L-Phe 37°C, pH 9.8 Onchocerca ochengi
3.33
-
HPO42- 37°C, pH 9.8 Onchocerca ochengi