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Literature summary for 3.1.3.1 extracted from

  • Choi, J.J.; Park, J.W.; Shim, H.; Lee, S.; Kwon, M.; Yang, J.; Hwang, H.; Kwon, S.
    Cloning, expression, and characterization of a hyperalkaline phosphatase from the thermophilic bacterium Thermus sp. T351 (2006), J. Microbiol. Biotechnol., 16, 272-279.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21(DE3) Thermus sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
4-nitrophenyl phosphate 80°C, pH 12 Thermus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, activates Thermus sp.
Mg2+ 1 mM, 10fold activation Thermus sp.
Mn2+ 1 mM, slight activation Thermus sp.
Zn2+ 1 mM, slight activation Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
x * 52000, SDS-PAGE Thermus sp.
54407
-
x * 54407, calculated from sequence Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp. B8K1W1
-
-
Thermus sp. T351 B8K1W1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Thermus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.8
-
-
Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine 5'-monophosphate + H2O 113.3% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
2'-deoxyadenosine 5'-monophosphate + H2O 113.3% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
?
4-nitrophenyl phosphate + H2O
-
Thermus sp. 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Thermus sp. T351 4-nitrophenol + phosphate
-
?
5-bromo-4-chloro-3-indolyl phosphate + H2O 50.3% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
5-bromo-4-chloro-3-indolyl phosphate + H2O 50.3% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
?
adenosine 5'-monophosphate + H2O 306.1% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
adenosine 5'-triphosphate + H2O 257.4% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
alpha-naphthyl acid phosphate + H2O 88.6% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
bis(p-nitrophenyl) phosphate + H2O 6.1% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
bis(p-nitrophenyl) phosphate + H2O 6.1% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
?
cytidine 5'-monophosphate + H2O 343.8% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
glycerophosphate + H2O 248.7% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
glycerophosphate + H2O 248.7% of the activity with 4-nitrophenyl phosphate Thermus sp. T351 ?
-
?
guanosine 5'-monophosphate + H2O 358.8% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?
uridine 5'-monophosphate + H2O 388.6% of the activity with 4-nitrophenyl phosphate Thermus sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Thermus sp.
? x * 54407, calculated from sequence Thermus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: aout 50% of maximal activity, 90°C: about 40% of maximal activity Thermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
half-life is 103 min Thermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
12
-
-
Thermus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 12 25°C, 16 h, stable Thermus sp.