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Literature summary for 3.1.3.1 extracted from

  • Helianti, I.; Okubo, T.; Morita, Y.; Tamiya, E.
    Characterization of thermostable native alkaline phosphatase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 (2007), Appl. Microbiol. Biotechnol., 74, 107-112.
    View publication on PubMed
Search for more literature for 3.1.3.1

General Stability

General Stability Organism
Mg2+ increases stability Aeropyrum pernix

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2 mM, 21% inhibition Aeropyrum pernix
Mn2+ 2 mM, 24% inhibition Aeropyrum pernix

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 2 mM, 2.2fold increase in activity Aeropyrum pernix
Ca2+ 2 mM, 4.95fold increase in activity Aeropyrum pernix
Co2+ 2 mM, 2.6fold increase in activity Aeropyrum pernix
Fe2+ 2 mM, 2.8fold increase in activity Aeropyrum pernix
Mg2+ 2 mM, 4.3fold increase in activity Aeropyrum pernix
Ni2+ 2 mM, 1-3fold increase in activity Aeropyrum pernix
Sr2+ 2 mM, 4fold increase in activity Aeropyrum pernix
Zn2+ 2 mM, 2.1fold increase in activity Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Aeropyrum pernix
75000
-
 gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeropyrum pernix

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
427
-
-
 Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Aeropyrum pernix 4-nitrophenol + phosphate
-
 ?
adenosine 5'-diphosphate + H2O 77% of the activity with 4-nitrophenyl phosphate Aeropyrum pernix AMP + phosphate
-
 ?
adenosine 5'-triphosphate + H2O 61% of the activity with 4-nitrophenyl phosphate Aeropyrum pernix AMP + phosphate
-
 ?
D-fructose 1,6-diphosphate + H2O 52% of the activity with 4-nitrophenyl phosphate Aeropyrum pernix ?
-
 ?
D-fructose 6-phosphate + H2O 36% of the activity with 4-nitrophenyl phosphate Aeropyrum pernix D-fructose + phosphate
-
 ?
D-glucose 1-phosphate + H2O 32% of the activity with 4-nitrophenyl phosphate Aeropyrum pernix D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 60% of the activity with 4-nitrophenyl phosphate Aeropyrum pernix D-glucose + phosphate
-
 ?
glycerophosphate + H2O 63% of the activity with 4-nitrophenyl phosphate Aeropyrum pernix glycerol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 5.5 h, 15% loss of activity Aeropyrum pernix
90
-
 5.5 h, 24% loss of activity. Half-life: 562 min Aeropyrum pernix
100
-
 2.5 h, 33% loss of activity. Half-life: 363 min Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Aeropyrum pernix

pH Range

pH Minimum pH Maximum Comment Organism
8 11 pH 8.0: about 70% of maximal activity, pH 11.0: about 30% of maximal activity Aeropyrum pernix