Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 70% decrease in activity | Cobetia marina | |
Cd2+ | 5 mM, 30% inhibition | Cobetia marina | |
Co2+ | 5 mM, 60% inhibition | Cobetia marina | |
Cu2+ | 5 mM, 40% inhibition | Cobetia marina | |
dithiothreitol | 10 mM, 95% decrease in activity | Cobetia marina | |
glycerol | concentrations of 10%, 20% and 40% inhibit the activity by 35%, 60%, and 90% | Cobetia marina | |
additional information | iodoacetamide and NaN3 at 2-10 mM concentration have no effect on activity | Cobetia marina | |
Ni2+ | 5 mM, 27% inhibition | Cobetia marina | |
p-chloromercuribenzoate | 0.1-0.2 mM, 85%-100% inhibition | Cobetia marina | |
Zn2+ | 5 mM, complete inhibition | Cobetia marina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 0.1 M, stimulates | Cobetia marina | |
Na+ | 0.1 M, stimulates | Cobetia marina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, SDS-PAGE | Cobetia marina |
62000 | - |
gel filtration | Cobetia marina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cobetia marina | - |
- |
- |
Cobetia marina KMM MC-296 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cobetia marina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3-AMP + H2O | 20% of activity with p-nitrophenyl phosphate | Cobetia marina | ? | - |
? | |
5'-AMP + H2O | 90% of activity with p-nitrophenyl phosphate | Cobetia marina | adenosine + phosphate | - |
? | |
5'-AMP + H2O | 90% of activity with p-nitrophenyl phosphate | Cobetia marina KMM MC-296 | adenosine + phosphate | - |
? | |
5'-CMP + H2O | 80% of activity with p-nitrophenyl phosphate | Cobetia marina | cytidine + phosphate | - |
? | |
5'-dCMP + H2O | 80% of activity with p-nitrophenyl phosphate | Cobetia marina | deoxycytidine + phosphate | - |
? | |
5'-GMP + H2O | 40% of activity with p-nitrophenyl phosphate | Cobetia marina | guanosine + phosphate | - |
? | |
5'-UMP + H2O | 30% of activity with p-nitrophenyl phosphate | Cobetia marina | uridine + phosphate | - |
? | |
5'-UMP + H2O | 30% of activity with p-nitrophenyl phosphate | Cobetia marina KMM MC-296 | uridine + phosphate | - |
? | |
ADP + H2O | 20% of activity with p-nitrophenyl phosphate | Cobetia marina | ? | - |
? | |
ADP + H2O | 20% of activity with p-nitrophenyl phosphate | Cobetia marina KMM MC-296 | ? | - |
? | |
alpha-glycerophosphate + H2O | 60% of activity with p-nitrophenyl phosphate | Cobetia marina | glycerol + phosphate | - |
? | |
ATP + H2O | 10% of activity with p-nitrophenyl phosphate | Cobetia marina | ? | - |
? | |
ATP + H2O | 10% of activity with p-nitrophenyl phosphate | Cobetia marina KMM MC-296 | ? | - |
? | |
beta-glycerophosphate + H2O | 20% of activity with p-nitrophenyl phosphate | Cobetia marina | glycerol + phosphate | - |
? | |
beta-glycerophosphate + H2O | 20% of activity with p-nitrophenyl phosphate | Cobetia marina KMM MC-296 | glycerol + phosphate | - |
? | |
NADP+ + H2O | 20% of activity with p-nitrophenyl phosphate | Cobetia marina | ? | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Cobetia marina | p-nitrophenol + phosphate | - |
? | |
UDP-glucose + H2O | 20% of activity with p-nitrophenyl phosphate | Cobetia marina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Cobetia marina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to, 15 min | Cobetia marina |
55 | - |
15 min, about 20% loss of activity | Cobetia marina |
58 | - |
15 min, about 55% loss of activity | Cobetia marina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.3 | - |
- |
Cobetia marina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.9 | 10.7 | about 50% of maximal activity at pH 9.9 and pH 10.7 | Cobetia marina |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
15 min, 20% loss of activity | Cobetia marina |
6 | - |
15 min, 10% loss of activity | Cobetia marina |
7 | 11 | 15 min, stable | Cobetia marina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cobetia marina | chromatofocusing | - |
4.5 |