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Literature summary for 3.1.3.1 extracted from

  • Tan, A.S.; Worobec, E.A.
    Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa (1993), FEMS Microbiol. Lett., 106, 281-286.
    View publication on PubMed
Search for more literature for 3.1.3.1

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas aeruginosa
-
-
periplasm
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 1 * 39500, low-molecular weight alkaline phosphatase Pseudomonas aeruginosa
51000
-
 x * 51000, high-molecular weight alkaline phosphatase Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa a low level of high-molecular weight alkaline phosphatase is produced constitutively, low-molecular weight alkaline phosphatase is produced only after induction ?
-
 ?
additional information Pseudomonas aeruginosa H103 a low level of high-molecular weight alkaline phosphatase is produced constitutively, low-molecular weight alkaline phosphatase is produced only after induction ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 H103
-
Pseudomonas aeruginosa H103
-
 H103
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
 high-molecular weight alkaline phosphatase Pseudomonas aeruginosa
46
-
 low-molecular weight alkaline phosphatase Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a low level of high-molecular weight alkaline phosphatase is produced constitutively, low-molecular weight alkaline phosphatase is produced only after induction Pseudomonas aeruginosa ?
-
 ?
additional information high-molecular weight alkaline phosphatase is active as phosphomonoesterase, low-molecular weight alkaline phosphatase is active as phosphomonoesterase and phosphodiesterase Pseudomonas aeruginosa ?
-
 ?
additional information a low level of high-molecular weight alkaline phosphatase is produced constitutively, low-molecular weight alkaline phosphatase is produced only after induction Pseudomonas aeruginosa H103 ?
-
 ?
additional information high-molecular weight alkaline phosphatase is active as phosphomonoesterase, low-molecular weight alkaline phosphatase is active as phosphomonoesterase and phosphodiesterase Pseudomonas aeruginosa H103 ?
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa H103 p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, high-molecular weight alkaline phosphatase Pseudomonas aeruginosa
monomer 1 * 39500, low-molecular weight alkaline phosphatase Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
H-AP high-molecular weight alkaline phosphatase Pseudomonas aeruginosa
L-AP low-molecular weight alkaline phosphatase Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 low-molecular weight alkaline phosphatase, phosphodiesterase activity, CHES buffer Pseudomonas aeruginosa
10
-
 low-molecular weight alkaline phosphatase and high-molecular weight alkaline phosphatase, phosphomonoesterase activity Pseudomonas aeruginosa
10
-
 low-molecular weight alkaline phosphatase, phosphodiesterase activity, CAPS buffer Pseudomonas aeruginosa