Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Zn2+ and Mg2+ do not affect the mutant T59R enzyme | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression of the wild-type and mutant enzymes | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
T59A | site-directed mutagenesis, exists as a dimer, shows catalytic activity and metal content similar to the wild-type enzyme | Escherichia coli |
T59R | site-directed mutagenesis, exists as a monomer, shows 10000fold reduced activity compared to the wild-type, highly reduced metal content, highly reduced thermal stability | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Zn2+ and Mg2+ do not affect the mutant T59R enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0218 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Escherichia coli | |
0.0249 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59A | Escherichia coli | |
0.0392 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59R | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloenzyme, mutant T59A contains sn amount similar to the wild-type enzyme, while mutant T59R has almost undetectable amounts of bound metal | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
recombinant mutant T59R, sucrose density gradient sedimentation | Escherichia coli |
87000 | - |
recombinant wild-type and mutant T59A enzymes, sucrose density gradient sedimentation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00634 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Escherichia coli | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, SDS-PAGE, recombinant wild-type and mutant T59A | Escherichia coli |
monomer | 1 * 44000, SDS-PAGE, recombinant mutant T59R | Escherichia coli |
More | Thr59 is involved in stabilization of the dimeric interface, structural analysis | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Escherichia coli |
AP | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59R | Escherichia coli | |
1.5 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59A | Escherichia coli | |
2.3 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Escherichia coli | |
56.3 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant mutant T59A | Escherichia coli | |
74.7 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |