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Literature summary for 3.1.3.1 extracted from
- Differences in sialic acid residues among bone alkaline phosphatase isoforms: a physical, biochemical, and immunological characterization (2002), Calcif. Tissue Int., 71, 508-518.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-homoarginine | 83% inhibition at 10 mM for all three isozymes | Homo sapiens |  |
| levamisole | 96-98% inhibition at 1 mM for all three isozymes | Homo sapiens | |
| additional information | no inhibition at 10 mM by L-phenylalanine at 10 mM for all three isozymes | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens | |
| 0.028 | - |
4-nitrophenyl phosphate | isozyme B2, pH 8.8 | Homo sapiens | |
| 0.034 | - |
4-nitrophenyl phosphate | isozyme B1, pH 8.8 | Homo sapiens | |
| 0.038 | - |
4-nitrophenyl phosphate | isozyme B/I, pH 8.8 | Homo sapiens | |
| 0.32 | - |
4-nitrophenyl phosphate | isozyme B/I, pH 10.0 | Homo sapiens | |
| 0.35 | - |
4-nitrophenyl phosphate | isozyme B2, pH 10.0 | Homo sapiens | |
| 0.48 | - |
4-nitrophenyl phosphate | isozyme B1, pH 10.0 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | BALP is an ectoenzyme attached to the osteoblast cell membrane by a glycosylphosphatidylinositol anchor | Homo sapiens | - |
- |
| membrane | BALP is an ectoenzyme attached to the osteoblast cell membrane by a glycosylphosphatidylinositol anchor | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 126000 | - |
isozyme B/I, native PAGE | Homo sapiens |
| 136000 | - |
isozyme B1, native PAGE | Homo sapiens |
| 141000 | - |
isozyme B2, native PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme is necessary for the initiation of mineralization by osteoblast-derived matrix vesicles but not for the continuation of the processes | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosylated, isozymes B1, B2, and B/I, contain different amounts of carbohydrates with terminal sialic acids, total carbohydrate content of the isozymes is 14% for B/I, 21% for B1, and 23% for B2 | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification of isozymes | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone | - |
Homo sapiens | - |
| additional information | the different enzyme forms differ in their half-lives of circulation | Homo sapiens | - |
| osteoblast | - |
Homo sapiens | - |
| SAOS-2 cell | osteosarcoma cells, contain isozymes of the bone alkaline phosphatase form, main isozymes are B1 and B2, and intestinal alkaline phosphatase form, e.g. isozyme B/I which is composed of 70% bone and 30% intestinal enzyme | Homo sapiens | - |
| serum | contains isozymes of the bone alkaline phosphatase form, main isozymes are B1 and B2, and intestinal alkaline phosphatase form, e.g. isozyme B/I which is composed of 70% bone and 30% intestinal enzyme | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
| additional information | the enzyme is necessary for the initiation of mineralization by osteoblast-derived matrix vesicles but not for the continuation of the processes | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BALP | - |
Homo sapiens |
| bone alkaline phosphatase | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
15 min, 3-4% remaining activity for all three isozymes | Homo sapiens |
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