Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.27.7 extracted from

  • Watson, N.; Apirion, D.
    Ribonuclease F, a putative processing endoribonuclease from Escherichia coli (1981), Biochem. Biophys. Res. Commun., 103, 543-551.
    View publication on PubMed
Search for more literature for 3.1.27.7

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulating, optimum: 150-200 mM Escherichia coli
Na+ stimulating, optimum: 150-200 mM Escherichia coli
NH4+ stimulating, optimum: 150-200 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA precursors + H2O Escherichia coli
-
 3'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, 0.44 mg protein per ml, 1.5 ml aliquots Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA precursors + H2O
-
 Escherichia coli 3'-phosphooligonucleotides
-
 ?
tRNA precursors + H2O cleavage within a possible stem-loop at the border between ds and ss regions Escherichia coli 3'-phosphooligonucleotides
-
 ?
tRNA precursors + H2O cleavage near the 3'-end of tRNAs Escherichia coli 3'-phosphooligonucleotides
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 enzyme is active up to this temperature, above: spontaneous substrate-breakdown Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.6
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Escherichia coli