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Literature summary for 3.1.26.9 extracted from

  • Sana, B.; Ghosh, D.; Saha, M.; Mukherjee, J.
    Purification and characterization of an extracellular, uracil specific ribonuclease from a Bizionia species isolated from the marine environment of the Sundarbans (2008), Microbiol. Res., 163, 31-38.
    View publication on PubMed
Search for more literature for 3.1.26.9

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Bizionia sp.
Fe2+
-
 Bizionia sp.
Hg2+
-
 Bizionia sp.
human placental RNase inhibitor protein halves the RNase activity Bizionia sp.
additional information DTT and beta-mercaptoethanol do not inhibit the activity; no inhibition by dithiothreitol and beta-mercaptoethanol Bizionia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bizionia sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ enhances activity Bizionia sp.
Ca2+ enhances activity Bizionia sp.
Mg2+ enhances activity Bizionia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 SDS-PAGE Bizionia sp.

Organism

Organism UniProt Comment Textmining
Bizionia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bizionia sp.
enzyme is purified from Bizionia species. Extracellular RNase by using acetone precipitation, cation exchange chromatography, anion exchange chromatography and size exclusion chromatography. Enzyme is purified 29-fold with a recovery of 4% Bizionia sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bizionia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 630 unit/mg protein Bizionia sp.
additional information
-
 the enzyme retains more than half of its activity (relative to optimal assay conditions) after 1 h pre-incubation separately with 5% (w/v) NaCl or from pH 5.0 to 8.5 or at 50°C Bizionia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information RNase degrades uracil containing nucleic acids only Bizionia sp. ?
-
 ?
additional information RNase degrades only uracil containing nucleic acids Bizionia sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
ribonuclease
-
 Bizionia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Bizionia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, enzyme retains 50% of maximal activity Bizionia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bizionia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.5 1 h, enzyme retains 50% of maximal ativity Bizionia sp.