Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of ribozyme mutants with moved bulged nucleotides important for metal ion coordination, proximal or distal to metal coordination sites, the first has moderate but positive effect on catalysis and cleavage pattern, the latter has large, negative effects on catalysis and cleavage pattern, overview | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutant ribozymes | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, potential metal binding sites identification by Tb3+ cleavage | Escherichia coli | |
additional information | conservation of helical structure contributes to functional metal ion interactions in the catalytic domain of the ribozyme | Escherichia coli | |
NaCl | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
catalytic RNA | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of RNA, removing 3'-extranucleotides from tRNA precursor | cleavage sites and pattern | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | structure-function relationship, moving the bulged nucleotides proximal to sites of metal ion coordination in helix P4 is important for catalysis, overview | Escherichia coli | tRNA + pre-part nucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease P RNA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, standard trans-conditions | Escherichia coli |
50 | - |
assay at, standard cis-conditions | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at, standard cis-conditions | Escherichia coli |
6 | - |
assay at, standard trans-conditions | Escherichia coli |