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Literature summary for 3.1.26.5 extracted from
- Unexpected diversity of RNase P, an ancient tRNA processing enzyme: challenges and prospects (2010), FEBS Lett., 584, 287-296.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | coupling DNA micro-arrays with conditional mutants of RNase P in yeast is a powerful approach to conduct genomewide searches and identify new substrates (e.g., polycistronic mRNAs, snoRNAs) | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
| nucleus | - |
Homo sapiens | 5634 | - |
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity purification | Homo sapiens |
| affinity purified | Saccharomyces cerevisiae |
| partially purified | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | yeast RNase P may process antisense RNAs from genes encoding ribosomal proteins | Saccharomyces cerevisiae | ? | - |
? |  |
| pre-tRNA + H2O | - |
Homo sapiens | tRNA + 5'-oligoribonucleotide | - |
? | |
| pre-tRNA + H2O | the base at N-1 in the pre-tRNA interacts with A248 in the RNase P RNA | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MRPP1 | - |
Homo sapiens |
| MRPP2 | - |
Homo sapiens |
| MRPP3 | - |
Homo sapiens |
| POP1 | - |
Homo sapiens |
| Pop5 | - |
Homo sapiens |
| Pop5 | - |
Methanothermobacter thermautotrophicus |
| ribonuclease P | - |
Escherichia coli |
| ribonuclease P | - |
Homo sapiens |
| ribonuclease P | - |
Saccharomyces cerevisiae |
| ribonuclease P | - |
Methanothermobacter thermautotrophicus |
| RNase P | - |
Escherichia coli |
| RNase P | - |
Homo sapiens |
| RNase P | - |
Saccharomyces cerevisiae |
| RNase P | - |
Methanothermobacter thermautotrophicus |
| RPP14 | - |
Homo sapiens |
| RPP14 | - |
Saccharomyces cerevisiae |
| Rpp20 | - |
Homo sapiens |
| Rpp20 | - |
Saccharomyces cerevisiae |
| Rpp21 | - |
Homo sapiens |
| Rpp21 | - |
Saccharomyces cerevisiae |
| Rpp21 | - |
Methanothermobacter thermautotrophicus |
| Rpp25 | - |
Homo sapiens |
| Rpp25 | - |
Saccharomyces cerevisiae |
| Rpp29 | - |
Homo sapiens |
| Rpp29 | - |
Saccharomyces cerevisiae |
| Rpp29 | - |
Methanothermobacter thermautotrophicus |
| Rpp30 | - |
Homo sapiens |
| Rpp30 | - |
Saccharomyces cerevisiae |
| Rpp30 | - |
Methanothermobacter thermautotrophicus |
| Rpp38 | - |
Homo sapiens |
| Rpp38 | - |
Saccharomyces cerevisiae |
| RPP40 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | native RNase P turnover at steady state is limited by product release | Saccharomyces cerevisiae |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | down-regulation of a single RNase P protein in cultured human cells results in a concomitant decrease of up to four other RNase P proteins (but not the RNase P RNA), likely due to transcriptional repression | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inactivation of RNase P results in decreased transcription of several non-coding RNAs in a cell cycle-dependent fashion | Homo sapiens |
| physiological function | native nuclear RNase P has an RNase P RNA plus nine RNase P proteins. All subunits are essential for RNase P activity and cell viability. Only the nuclear-encoded RPM2 is known and shown genetically to be required for mitochondrial RNase P activity | Saccharomyces cerevisiae |
| physiological function | native nuclear RNase P has an RNase P RNA plus ten RNase P proteins. The protein-only mitochondrial RNase P is composed of three proteins (MRPP1-MRPP3). MRPP1, which methylates G9 of tRNAs, may be responsible for substrate recognition. RNase P RNA is weakly active without RNase P proteins, some activity is present when reconstituted with RPP21 and RPP29 | Homo sapiens |
| physiological function | the holoenzyme consists of a single RNase P RNA associated with RNase P protein subunits | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | a native precursor form of yeast RNase P (RNase P RNA + 7 RNase P proteins, without POP3/RPP38 and Rpr2p/RPP21) displays an identical steady-state rate as the mature form (RNase P RNA + 9 RNase P proteins) | Saccharomyces cerevisiae |
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